Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-153_S4_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-153_S4_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6130
Pairs without alignments under any condition 2055
Pairs that did not map uniquely 1815
Genomic sequence context not extractable (edges of chromosomes) 44

Cytosine Methylation

Total C's analysed 193775
Methylated C's in CpG context 6046
Methylated C's in CHG context 1009
Methylated C's in CHH context 11493
Methylated C's in Unknown context 372
Unmethylated C's in CpG context 22307
Unmethylated C's in CHG context 39851
Unmethylated C's in CHH context 113069
Unmethylated C's in Unknown context 1138
Percentage methylation (CpG context) 21.3%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 9.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2652 original top strand
CTOT 259 complementary to original top strand
CTOB 272 complementary to original bottom strand
OB 2903 original bottom strand

Deduplication

Alignments analysed 6086
Unique alignments 6020
Duplicates removed 66
Duplicated alignments were found at 30 different positions

Cytosine Methylation after Extraction

Total C's analysed 135404
Methylated C's in CpG context 4296
Methylated C's in CHG context 766
Methylated C's in CHH context 8678
Unmethylated C's in CpG context 15179
Unmethylated C's in CHG context 27307
Unmethylated C's in CHH context 79178
Percentage methylation (CpG context) 22.1%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 9.9%

M-Bias Plot