Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-152_S3_L002_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-152_S3_L002_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 5756
Pairs without alignments under any condition 2446
Pairs that did not map uniquely 1798
Genomic sequence context not extractable (edges of chromosomes) 30

Cytosine Methylation

Total C's analysed 182157
Methylated C's in CpG context 5913
Methylated C's in CHG context 975
Methylated C's in CHH context 9229
Methylated C's in Unknown context 296
Unmethylated C's in CpG context 19371
Unmethylated C's in CHG context 36413
Unmethylated C's in CHH context 110256
Unmethylated C's in Unknown context 1003
Percentage methylation (CpG context) 23.4%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2618 original top strand
CTOT 206 complementary to original top strand
CTOB 216 complementary to original bottom strand
OB 2686 original bottom strand

Deduplication

Alignments analysed 5726
Unique alignments 5685
Duplicates removed 41
Duplicated alignments were found at 25 different positions

Cytosine Methylation after Extraction

Total C's analysed 130451
Methylated C's in CpG context 4328
Methylated C's in CHG context 732
Methylated C's in CHH context 6589
Unmethylated C's in CpG context 13789
Unmethylated C's in CHG context 25759
Unmethylated C's in CHH context 79254
Percentage methylation (CpG context) 23.9%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 7.7%

M-Bias Plot