Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-143_S37_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-143_S37_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6311
Pairs without alignments under any condition 1939
Pairs that did not map uniquely 1750
Genomic sequence context not extractable (edges of chromosomes) 10

Cytosine Methylation

Total C's analysed 198698
Methylated C's in CpG context 6018
Methylated C's in CHG context 973
Methylated C's in CHH context 7682
Methylated C's in Unknown context 306
Unmethylated C's in CpG context 21136
Unmethylated C's in CHG context 40401
Unmethylated C's in CHH context 122488
Unmethylated C's in Unknown context 1029
Percentage methylation (CpG context) 22.2%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 5.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2945 original top strand
CTOT 185 complementary to original top strand
CTOB 167 complementary to original bottom strand
OB 3004 original bottom strand

Deduplication

Alignments analysed 6301
Unique alignments 6248
Duplicates removed 53
Duplicated alignments were found at 36 different positions

Cytosine Methylation after Extraction

Total C's analysed 148693
Methylated C's in CpG context 4656
Methylated C's in CHG context 770
Methylated C's in CHH context 5761
Unmethylated C's in CpG context 15651
Unmethylated C's in CHG context 29822
Unmethylated C's in CHH context 92033
Percentage methylation (CpG context) 22.9%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 5.9%

M-Bias Plot