Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-136_S36_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-136_S36_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6232
Pairs without alignments under any condition 1933
Pairs that did not map uniquely 1835
Genomic sequence context not extractable (edges of chromosomes) 22

Cytosine Methylation

Total C's analysed 195193
Methylated C's in CpG context 5275
Methylated C's in CHG context 935
Methylated C's in CHH context 6737
Methylated C's in Unknown context 256
Unmethylated C's in CpG context 21796
Unmethylated C's in CHG context 39263
Unmethylated C's in CHH context 121187
Unmethylated C's in Unknown context 1018
Percentage methylation (CpG context) 19.5%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 5.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2897 original top strand
CTOT 170 complementary to original top strand
CTOB 199 complementary to original bottom strand
OB 2944 original bottom strand

Deduplication

Alignments analysed 6210
Unique alignments 6113
Duplicates removed 97
Duplicated alignments were found at 41 different positions

Cytosine Methylation after Extraction

Total C's analysed 141866
Methylated C's in CpG context 3921
Methylated C's in CHG context 732
Methylated C's in CHH context 5272
Unmethylated C's in CpG context 15676
Unmethylated C's in CHG context 28147
Unmethylated C's in CHH context 88118
Percentage methylation (CpG context) 20.0%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 5.6%

M-Bias Plot