Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-135_S35_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-135_S35_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6330
Pairs without alignments under any condition 1708
Pairs that did not map uniquely 1962
Genomic sequence context not extractable (edges of chromosomes) 22

Cytosine Methylation

Total C's analysed 195995
Methylated C's in CpG context 5649
Methylated C's in CHG context 1064
Methylated C's in CHH context 8266
Methylated C's in Unknown context 350
Unmethylated C's in CpG context 21802
Unmethylated C's in CHG context 38695
Unmethylated C's in CHH context 120519
Unmethylated C's in Unknown context 1134
Percentage methylation (CpG context) 20.6%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 6.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2909 original top strand
CTOT 253 complementary to original top strand
CTOB 219 complementary to original bottom strand
OB 2927 original bottom strand

Deduplication

Alignments analysed 6308
Unique alignments 6204
Duplicates removed 104
Duplicated alignments were found at 53 different positions

Cytosine Methylation after Extraction

Total C's analysed 142620
Methylated C's in CpG context 4259
Methylated C's in CHG context 861
Methylated C's in CHH context 6377
Unmethylated C's in CpG context 15531
Unmethylated C's in CHG context 27686
Unmethylated C's in CHH context 87906
Percentage methylation (CpG context) 21.5%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 6.8%

M-Bias Plot