Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-128_S34_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-128_S34_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6564
Pairs without alignments under any condition 1667
Pairs that did not map uniquely 1769
Genomic sequence context not extractable (edges of chromosomes) 25

Cytosine Methylation

Total C's analysed 211134
Methylated C's in CpG context 6362
Methylated C's in CHG context 1036
Methylated C's in CHH context 7106
Methylated C's in Unknown context 257
Unmethylated C's in CpG context 23729
Unmethylated C's in CHG context 42062
Unmethylated C's in CHH context 130839
Unmethylated C's in Unknown context 1050
Percentage methylation (CpG context) 21.1%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 5.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3060 original top strand
CTOT 159 complementary to original top strand
CTOB 158 complementary to original bottom strand
OB 3162 original bottom strand

Deduplication

Alignments analysed 6539
Unique alignments 6450
Duplicates removed 89
Duplicated alignments were found at 55 different positions

Cytosine Methylation after Extraction

Total C's analysed 157580
Methylated C's in CpG context 4920
Methylated C's in CHG context 809
Methylated C's in CHH context 5464
Unmethylated C's in CpG context 17284
Unmethylated C's in CHG context 31012
Unmethylated C's in CHH context 98091
Percentage methylation (CpG context) 22.2%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 5.3%

M-Bias Plot