Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-127_S33_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-127_S33_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6581
Pairs without alignments under any condition 1609
Pairs that did not map uniquely 1810
Genomic sequence context not extractable (edges of chromosomes) 11

Cytosine Methylation

Total C's analysed 211984
Methylated C's in CpG context 6231
Methylated C's in CHG context 1042
Methylated C's in CHH context 7448
Methylated C's in Unknown context 236
Unmethylated C's in CpG context 23778
Unmethylated C's in CHG context 42363
Unmethylated C's in CHH context 131122
Unmethylated C's in Unknown context 976
Percentage methylation (CpG context) 20.8%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 5.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3113 original top strand
CTOT 157 complementary to original top strand
CTOB 159 complementary to original bottom strand
OB 3141 original bottom strand

Deduplication

Alignments analysed 6570
Unique alignments 6493
Duplicates removed 77
Duplicated alignments were found at 45 different positions

Cytosine Methylation after Extraction

Total C's analysed 159190
Methylated C's in CpG context 4828
Methylated C's in CHG context 811
Methylated C's in CHH context 5724
Unmethylated C's in CpG context 17628
Unmethylated C's in CHG context 31434
Unmethylated C's in CHH context 98765
Percentage methylation (CpG context) 21.5%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 5.5%

M-Bias Plot