Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-120_S32_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6512
Pairs without alignments under any condition 1703
Pairs that did not map uniquely 1785
Genomic sequence context not extractable (edges of chromosomes) 28

Cytosine Methylation

Total C's analysed 207395
Methylated C's in CpG context 6206
Methylated C's in CHG context 1007
Methylated C's in CHH context 7369
Methylated C's in Unknown context 220
Unmethylated C's in CpG context 21912
Unmethylated C's in CHG context 41660
Unmethylated C's in CHH context 129241
Unmethylated C's in Unknown context 987
Percentage methylation (CpG context) 22.1%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 5.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3090 original top strand
CTOT 158 complementary to original top strand
CTOB 153 complementary to original bottom strand
OB 3083 original bottom strand

Deduplication

Alignments analysed 6484
Unique alignments 6411
Duplicates removed 73
Duplicated alignments were found at 29 different positions

Cytosine Methylation after Extraction

Total C's analysed 154900
Methylated C's in CpG context 4750
Methylated C's in CHG context 793
Methylated C's in CHH context 5585
Unmethylated C's in CpG context 16419
Unmethylated C's in CHG context 30733
Unmethylated C's in CHH context 96620
Percentage methylation (CpG context) 22.4%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 5.5%

M-Bias Plot