Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-113_S30_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-113_S30_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6583
Pairs without alignments under any condition 1673
Pairs that did not map uniquely 1744
Genomic sequence context not extractable (edges of chromosomes) 26

Cytosine Methylation

Total C's analysed 211918
Methylated C's in CpG context 5728
Methylated C's in CHG context 1046
Methylated C's in CHH context 7058
Methylated C's in Unknown context 257
Unmethylated C's in CpG context 24472
Unmethylated C's in CHG context 42311
Unmethylated C's in CHH context 131303
Unmethylated C's in Unknown context 1148
Percentage methylation (CpG context) 19.0%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 5.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3007 original top strand
CTOT 192 complementary to original top strand
CTOB 150 complementary to original bottom strand
OB 3208 original bottom strand

Deduplication

Alignments analysed 6557
Unique alignments 6426
Duplicates removed 131
Duplicated alignments were found at 63 different positions

Cytosine Methylation after Extraction

Total C's analysed 156309
Methylated C's in CpG context 4466
Methylated C's in CHG context 810
Methylated C's in CHH context 5412
Unmethylated C's in CpG context 17609
Unmethylated C's in CHG context 30781
Unmethylated C's in CHH context 97231
Percentage methylation (CpG context) 20.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 5.3%

M-Bias Plot