Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-111_S29_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6440
Pairs without alignments under any condition 1698
Pairs that did not map uniquely 1862
Genomic sequence context not extractable (edges of chromosomes) 27

Cytosine Methylation

Total C's analysed 200670
Methylated C's in CpG context 5920
Methylated C's in CHG context 989
Methylated C's in CHH context 6386
Methylated C's in Unknown context 270
Unmethylated C's in CpG context 21438
Unmethylated C's in CHG context 40265
Unmethylated C's in CHH context 125672
Unmethylated C's in Unknown context 1005
Percentage methylation (CpG context) 21.6%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 4.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3050 original top strand
CTOT 176 complementary to original top strand
CTOB 172 complementary to original bottom strand
OB 3015 original bottom strand

Deduplication

Alignments analysed 6413
Unique alignments 6345
Duplicates removed 68
Duplicated alignments were found at 32 different positions

Cytosine Methylation after Extraction

Total C's analysed 146763
Methylated C's in CpG context 4539
Methylated C's in CHG context 762
Methylated C's in CHH context 4818
Unmethylated C's in CpG context 15475
Unmethylated C's in CHG context 29050
Unmethylated C's in CHH context 92119
Percentage methylation (CpG context) 22.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 5.0%

M-Bias Plot