Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R1_001_val_1.fq.gz and /gscratch/srlab/strigg/data/Pgenr/FASTQS/EPI-104_S28_L005_R2_001_val_2.fq.gz

Data processed at 17:55 on 2018-10-25


Alignment Stats

Sequence pairs analysed in total 10000
Paired-end alignments with a unique best hit 6548
Pairs without alignments under any condition 1621
Pairs that did not map uniquely 1831
Genomic sequence context not extractable (edges of chromosomes) 14

Cytosine Methylation

Total C's analysed 205827
Methylated C's in CpG context 6353
Methylated C's in CHG context 952
Methylated C's in CHH context 7875
Methylated C's in Unknown context 257
Unmethylated C's in CpG context 21220
Unmethylated C's in CHG context 41078
Unmethylated C's in CHH context 128349
Unmethylated C's in Unknown context 1030
Percentage methylation (CpG context) 23.0%
Percentage methylation (CHG context) 2.3%
Percentage methylation (CHH context) 5.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3080 original top strand
CTOT 161 complementary to original top strand
CTOB 202 complementary to original bottom strand
OB 3091 original bottom strand

Deduplication

Alignments analysed 6534
Unique alignments 6453
Duplicates removed 81
Duplicated alignments were found at 32 different positions

Cytosine Methylation after Extraction

Total C's analysed 150459
Methylated C's in CpG context 4789
Methylated C's in CHG context 739
Methylated C's in CHH context 5848
Unmethylated C's in CpG context 15379
Unmethylated C's in CHG context 29587
Unmethylated C's in CHH context 94117
Percentage methylation (CpG context) 23.7%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 5.9%

M-Bias Plot