FastQCFastQC Report
Fri 13 Mar 2020
Meth5_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth5_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284116370
Sequences flagged as poor quality0
Sequence length20-150
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17787670.6260698741153141No Hit
TGGATTGAAGTTTATTTGGATTTGTGTTATTTGTTGGTGGTGTTAAAAGG10205400.3591978878232184No Hit
TGGAATATTAGTATTTTGGGTTTAATTTTTGTGTGGTTAGTGTTAAAAAA9118780.3209522914853516No Hit
AACCAAAATTCACTTAAATCCATACTACCTACTAATAATATTAAAAAAAT7836040.27580388979346737No Hit
AAACATCAACATCTCAAATCCAATCCTCACACAACCAACACCAAAAAACC2847000.10020541934982487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)