FastQCFastQC Report
Fri 13 Mar 2020
Meth5_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth5_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284116370
Sequences flagged as poor quality0
Sequence length20-150
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATATTAGTATTTTGGGTTTAATTTTTGTGTGGTTAGTGTTAAAAAA9360440.32945796118681936No Hit
TGGATTGAAGTTTATTTGGATTTGTGTTATTTGTTGGTGGTGTTAAAAGG9037000.31807389345429127No Hit
AACCAAAATTCACTTAAATCCATACTACCTACTAATAATATTAAAAAAAT8324870.29300916381551684No Hit
AAACATCAACATCTCAAATCCAATCCTCACACAACCAACACCAAAAAACC3336240.11742512407855979No Hit
AAACATCAACATCTCAAATCCAATCCTCACACAACCAACACCAAAAAACA3285840.11565120306161873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)