FastQCFastQC Report
Fri 13 Mar 2020
Meth4_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth4_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149828789
Sequences flagged as poor quality0
Sequence length20-150
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10874410.7257890871693558No Hit
TGGAATATTAGTATTTTGGGTTTAATTTTTGTGTGGTTAGTGTTAAAAAA4835630.3227437151614434No Hit
AAACATCAACATCTCAAATCCAATCCTCACACAACCAACACCAAAAAACC1698890.11338875601537432No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)