FastQCFastQC Report
Fri 13 Mar 2020
Meth15_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth15_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108911247
Sequences flagged as poor quality0
Sequence length20-150
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7392660.6787783818139553No Hit
TGGGAGGTAAGGTTGGTAGTAGAAAGTAGTGAAGGGTTAATTGTGTTTAA3957890.3634050760616119No Hit
AAATTTCAACCCATACAATCACTTTTAAACACAATTAACCCTTCACTACTTTCTACTACCAACCTTACCTCCCA3256440.2989994228970677No Hit
TGGGATTTTGGTTTGTGTGATTGTTTTTGGATGTAGTTGGTTTTTTGTTG3253970.2987726327291065No Hit
AAAAACAAAATCAATAACAAAAAACAACAAAAAACCAACTACATCCAAAAACAATCACACAAACCAAAATCCCA2733040.25094194358090494No Hit
AAATAACTCATTTAATACAACACTCCAACACCA1896940.17417301263661045No Hit
TGGTGTTGGAGTGTTGTATTAAATGAGTTATTTCG1887370.17329431550811278No Hit
ACACTAAAACATCATACCAAACAAATCATCCCA1806140.16583594897228565No Hit
TGGGATGATTTGTTTGGTATGATGTTTTAGTGTCG1750660.16074189289192511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)