FastQCFastQC Report
Fri 13 Mar 2020
Meth15_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth15_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108911247
Sequences flagged as poor quality0
Sequence length20-150
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTTCAACCCATACAATCACTTTTAAACACAATTAACCCTTCACTACTTTCTACTACCAACCTTACCTCCCA3707570.34042122389802404No Hit
TGGGAGGTAAGGTTGGTAGTAGAAAGTAGTGAAGGGTTAATTGTGTTTAAAAGTGATTGTATGGGTTGAAATTT3299760.30297697353515746No Hit
AAAAACAAAATCAATAACAAAAAACAACAAAAAACCAACTACATCCAAAAACAATCACACAAACCAAAATCCCA3003770.2757997987113305No Hit
TGGGATTTTGGTTTGTGTGATTGTTTTTGGATGTAGTTGGTTTTTTGTTGTTTTTTGTTATTGATTTTGTTTTT2761890.2535908894698451No Hit
AAATAACTCATTTAATACAACACTCCAACACCA2003770.18398191694563923No Hit
TGGTGTTGGAGTGTTGTATTAAATGAGTTATTT1925970.17683848574426847No Hit
ACACTAAAACATCATACCAAACAAATCATCCCA1856070.17042041580884662No Hit
TGGGATGATTTGTTTGGTATGATGTTTTAGTGT1832280.16823606840164082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)