FastQCFastQC Report
Thu 12 Mar 2020
Meth14_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth14_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69159859
Sequences flagged as poor quality0
Sequence length20-150
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4131810.5974289218837187No Hit
AAATTTCAACCCATACAATCACTTTTAAACACAATTAACCCTTCACTACTTTCTACTACCAACCTTACCTCCCA2771350.40071654859793737No Hit
TGGGAGGTAAGGTTGGTAGTAGAAAGTAGTGAAGGGTTAATTGTGTTTAA2418480.3496941773695635No Hit
AAAAACAAAATCAATAACAAAAAACAACAAAAAACCAACTACATCCAAAAACAATCACACAAACCAAAATCCCA2304450.333206289503858No Hit
TGGGATTTTGGTTTGTGTGATTGTTTTTGGATGTAGTTGGTTTTTTGTTG1961770.283657316305402No Hit
AAATAACTCATTTAATACAACACTCCAACACCA1554080.2247083817796679No Hit
ACACTAAAACATCATACCAAACAAATCATCCCA1507940.2180368817698139No Hit
TGGTGTTGGAGTGTTGTATTAAATGAGTTATTTCG1057990.15297746630744288No Hit
TGGGATGATTTGTTTGGTATGATGTTTTAGTGTCG998690.14440312841007963No Hit
ATTTTTTACATCACAAAAATCTAAAATAATTTAACTTTCCATCCATACTA966010.139677844051128No Hit
TGGATGAAAAAATATTTATTAGATGAGAAATGATAATTTTTTTAAAAGTG882280.12757111028812246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)