FastQCFastQC Report
Thu 12 Mar 2020
Meth14_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth14_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69159859
Sequences flagged as poor quality0
Sequence length20-150
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGAGGTAAGGTTGGTAGTAGAAAGTAGTGAAGGGTTAATTGTGTTTAAAAGTGATTGTATGGGTTGAAATTT2803860.40541725222429964No Hit
TGGGATTTTGGTTTGTGTGATTGTTTTTGGATGTAGTTGGTTTTTTGTTGTTTTTTGTTATTGATTTTGTTTTT2326580.33640612251682006No Hit
AAATTTCAACCCATACAATCACTTTTAAACACAATTAACCCTTCACTACTTTCTACTACCAACCTTACCTCCCA2282480.33002959129803894No Hit
AAAAACAAAATCAATAACAAAAAACAACAAAAAACCAACTACATCCAAAAACAATCACACAAACCAAAATCCCA1814440.2623544967030658No Hit
TGGTGTTGGAGTGTTGTATTAAATGAGTTATTT1574990.2277318118881648No Hit
TGGGATGATTTGTTTGGTATGATGTTTTAGTGT1529050.2210892303872395No Hit
AAATAACTCATTTAATACAACACTCCAACACCA1120010.16194509592623663No Hit
TGGATGAAAAAATATTTATTAGATGAGAAATGATAATTTTTTTAAAAGTG1073830.15526781221459693No Hit
ACACTAAAACATCATACCAAACAAATCATCCCA1060470.15333605581815893No Hit
ATTTTTTACATCACAAAAATCTAAAATAATTTAACTTTCCATCCATACTA837290.12106589170460859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)