FastQCFastQC Report
Thu 12 Mar 2020
Meth13_R2_001_val_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth13_R2_001_val_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100607927
Sequences flagged as poor quality0
Sequence length20-150
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5937500.5901622443726526No Hit
TGGGAGGTAAGGTTGGTAGTAGAAAGTAGTGAAGGGTTAATTGTGTTTAA3316200.3296161742801837No Hit
TGGGATTTTGGTTTGTGTGATTGTTTTTGGATGTAGTTGGTTTTTTGTTG2714570.2698167113611237No Hit
AAATTTCAACCCATACAATCACTTTTAAACACAATTAACCCTTCACTACTTTCTACTACCAACCTTACCTCCCA2711960.2695572884629657No Hit
AAAAACAAAATCAATAACAAAAAACAACAAAAAACCAACTACATCCAAAAACAATCACACAAACCAAAATCCCA2299980.22860822885258336No Hit
TGGTGTTGGAGTGTTGTATTAAATGAGTTATTTCG1580120.15705720683420898No Hit
AAATAACTCATTTAATACAACACTCCAACACCA1510880.15017504535204268No Hit
TGGGATGATTTGTTTGGTATGATGTTTTAGTGTCG1497870.14888190669110996No Hit
ACACTAAAACATCATACCAAACAAATCATCCCA1461720.14528875045800319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)