FastQCFastQC Report
Thu 12 Mar 2020
Meth13_R1_001_val_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMeth13_R1_001_val_1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100607927
Sequences flagged as poor quality0
Sequence length20-150
%GC22

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATTTCAACCCATACAATCACTTTTAAACACAATTAACCCTTCACTACTTTCTACTACCAACCTTACCTCCCA3072900.3054331891760378No Hit
TGGGAGGTAAGGTTGGTAGTAGAAAGTAGTGAAGGGTTAATTGTGTTTAAAAGTGATTGTATGGGTTGAAATTT2746170.27295761694801646No Hit
AAAAACAAAATCAATAACAAAAAACAACAAAAAACCAACTACATCCAAAAACAATCACACAAACCAAAATCCCA2519570.2504345408090955No Hit
TGGGATTTTGGTTTGTGTGATTGTTTTTGGATGTAGTTGGTTTTTTGTTGTTTTTTGTTATTGATTTTGTTTTT2321620.23075915280512638No Hit
AAATAACTCATTTAATACAACACTCCAACACCA1671570.1661469478443781No Hit
ACACTAAAACATCATACCAAACAAATCATCCCA1581970.1572410889650872No Hit
TGGTGTTGGAGTGTTGTATTAAATGAGTTATTT1532040.1522782593463038No Hit
TGGGATGATTTGTTTGGTATGATGTTTTAGTGT1482260.14733033908948348No Hit
TGGATGAAAAAATATTTATTAGATGAGAAATGATAATTTTTTTAAAAGTG1041310.10350178470529466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)