The exit status of the task that caused the workflow execution to fail was: 1.
The full error message was:
Error executing process > 'NFCORE_RNASPLICE:RNASPLICE:EDGER_DEU:EDGER_EXON (samplesheet.csv)'
Caused by:
Process `NFCORE_RNASPLICE:RNASPLICE:EDGER_DEU:EDGER_EXON (samplesheet.csv)` terminated with an error exit status (1)
Command executed:
run_edger_exon.R featurecounts samplesheet.csv contrastsheet.csv 10
cat <<-END_VERSIONS > versions.yml
"NFCORE_RNASPLICE:RNASPLICE:EDGER_DEU:EDGER_EXON":
r-base: $(echo $(R --version 2>&1) | sed 's/^.*R version //; s/ .*$//')
bioconductor-edger: $(Rscript -e "library(edgeR); cat(as.character(packageVersion('edgeR')))")
END_VERSIONS
Command exit status:
1
Command output:
(empty)
Command error:
INFO: Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred
INFO: Environment variable SINGULARITYENV_NXF_TASK_WORKDIR is set, but APPTAINERENV_NXF_TASK_WORKDIR is preferred
INFO: Environment variable SINGULARITYENV_NXF_DEBUG is set, but APPTAINERENV_NXF_DEBUG is preferred
Loading required package: limma
Error in eval(ej, envir = levelsenv) : object 'TREATMENT' not found
Calls: makeContrasts -> eval -> eval
Execution halted
Work dir:
/mmfs1/gscratch/scrubbed/strigg/analyses/20251205_rnasplice_Ptua/work/ef/6d347f430095e18b6e580c07b7a0a6
Container:
/gscratch/scrubbed/srlab/.apptainer/depot.galaxyproject.org-singularity-mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1-709335c37934db1b481054cbec637c6e5b5971cb-0.img
Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line
nextflow run nf-core/rnasplice -resume -c /gscratch/srlab/strigg/bin/uw_hyak_srlab.config --input samplesheet.csv --outdir /gscratch/scrubbed/strigg/analyses/20251205_rnasplice_Ptua --source genome_bam --contrasts contrastsheet.csv --skip_alignment --fasta /gscratch/srlab/strigg/GENOMES/Pocillopora_meandrina_HIv1.assembly.fasta --gtf /gscratch/srlab/strigg/GENOMES/Pocillopora_meandrina_HIv1.genes-validated.gtf93d808d3e7ec52e0026e14613cc4d4c92441b244-6514-4bb3-99ac-b47dc4b64997https://github.com/nf-core/rnasplice, revision master (commit hash 1d0494ae3402d1a46e0adadad24f81a0ff855c77)These plots give an overview of the distribution of resource usage for each process.
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