Nextflow workflow report

[fervent_newton]

Workflow execution completed unsuccessfully!

The exit status of the task that caused the workflow execution to fail was: 1.

The full error message was:

Error executing process > 'NFCORE_RNASPLICE:RNASPLICE:EDGER_DEU:EDGER_EXON (samplesheet.csv)'

Caused by:
  Process `NFCORE_RNASPLICE:RNASPLICE:EDGER_DEU:EDGER_EXON (samplesheet.csv)` terminated with an error exit status (1)


Command executed:

  run_edger_exon.R featurecounts samplesheet.csv contrastsheet.csv 10
  
  cat <<-END_VERSIONS > versions.yml
  "NFCORE_RNASPLICE:RNASPLICE:EDGER_DEU:EDGER_EXON":
      r-base: $(echo $(R --version 2>&1) | sed 's/^.*R version //; s/ .*$//')
      bioconductor-edger:  $(Rscript -e "library(edgeR); cat(as.character(packageVersion('edgeR')))")
  END_VERSIONS

Command exit status:
  1

Command output:
  (empty)

Command error:
  INFO:    Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred
  INFO:    Environment variable SINGULARITYENV_NXF_TASK_WORKDIR is set, but APPTAINERENV_NXF_TASK_WORKDIR is preferred
  INFO:    Environment variable SINGULARITYENV_NXF_DEBUG is set, but APPTAINERENV_NXF_DEBUG is preferred
  Loading required package: limma
  Error in eval(ej, envir = levelsenv) : object 'TREATMENT' not found
  Calls: makeContrasts -> eval -> eval
  Execution halted

Work dir:
  /mmfs1/gscratch/scrubbed/strigg/analyses/20251205_rnasplice_Ptua/work/ef/6d347f430095e18b6e580c07b7a0a6

Container:
  /gscratch/scrubbed/srlab/.apptainer/depot.galaxyproject.org-singularity-mulled-v2-419bd7f10b2b902489ac63bbaafc7db76f8e0ae1-709335c37934db1b481054cbec637c6e5b5971cb-0.img

Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line
Run times
10-Dec-2025 10:49:06 - 10-Dec-2025 11:02:49 (duration: 13m 43s)
  246 succeeded  
  0 cached  
  0 ignored  
  1 failed (3 retries)  
Nextflow command
nextflow run nf-core/rnasplice -resume -c /gscratch/srlab/strigg/bin/uw_hyak_srlab.config --input samplesheet.csv --outdir /gscratch/scrubbed/strigg/analyses/20251205_rnasplice_Ptua --source genome_bam --contrasts contrastsheet.csv --skip_alignment --fasta /gscratch/srlab/strigg/GENOMES/Pocillopora_meandrina_HIv1.assembly.fasta --gtf /gscratch/srlab/strigg/GENOMES/Pocillopora_meandrina_HIv1.genes-validated.gtf
CPU-Hours
8.2 (1% failed)
Launch directory
/mmfs1/gscratch/scrubbed/strigg/analyses/20251205_rnasplice_Ptua
Work directory
/mmfs1/gscratch/scrubbed/strigg/analyses/20251205_rnasplice_Ptua/work
Project directory
/mmfs1/gscratch/srlab/nextflow/bin/assets/nf-core/rnasplice
Script name
main.nf
Script ID
93d808d3e7ec52e0026e14613cc4d4c9
Workflow session
2441b244-6514-4bb3-99ac-b47dc4b64997
Workflow repository
https://github.com/nf-core/rnasplice, revision master (commit hash 1d0494ae3402d1a46e0adadad24f81a0ff855c77)
Workflow profile
standard
Nextflow version
version 25.04.6, build 5954 (01-07-2025 11:27 UTC)

Resource Usage

These plots give an overview of the distribution of resource usage for each process.

CPU

Memory

Job Duration

I/O

Tasks

This table shows information about each task in the workflow. Use the search box on the right to filter rows for specific values. Clicking headers will sort the table by that value and scrolling side to side will reveal more columns.

(tasks table omitted because the dataset is too big)