The exit status of the task that caused the workflow execution to fail was: 255
.
The full error message was:
Error executing process > 'NFCORE_RNAVAR:RNAVAR:VCF_ANNOTATE_ALL:VCF_ANNOTATE_SNPEFF:SNPEFF_SNPEFF (POC-201-TP1)' Caused by: Process `NFCORE_RNAVAR:RNAVAR:VCF_ANNOTATE_ALL:VCF_ANNOTATE_SNPEFF:SNPEFF_SNPEFF (POC-201-TP1)` terminated with an error exit status (255) Command executed: snpEff \ -Xmx88473M \ pmeandrina1 \ -nodownload -canon -v \ -csvStats POC-201-TP1.haplotypecaller.filtered_snpEff.csv \ -dataDir ${PWD}/data \ POC-201-TP1.haplotypecaller.filtered.vcf.gz \ > POC-201-TP1.haplotypecaller.filtered_snpEff.ann.vcf cat <<-END_VERSIONS > versions.yml "NFCORE_RNAVAR:RNAVAR:VCF_ANNOTATE_ALL:VCF_ANNOTATE_SNPEFF:SNPEFF_SNPEFF": snpeff: $(echo $(snpEff -version 2>&1) | cut -f 2 -d ' ') END_VERSIONS Command exit status: 255 Command output: (empty) Command error: INFO: Environment variable SINGULARITYENV_TMPDIR is set, but APPTAINERENV_TMPDIR is preferred INFO: Environment variable SINGULARITYENV_NXF_TASK_WORKDIR is set, but APPTAINERENV_NXF_TASK_WORKDIR is preferred INFO: Environment variable SINGULARITYENV_NXF_DEBUG is set, but APPTAINERENV_NXF_DEBUG is preferred 00:00:00 SnpEff version SnpEff 5.1d (build 2022-04-19 15:49), by Pablo Cingolani 00:00:00 Command: 'ann' 00:00:00 Reading configuration file 'snpEff.config'. Genome: 'pmeandrina1' 00:00:00 Reading config file: /gscratch/scrubbed/srlab/nxf.F0LtIcqUKo/snpEff.config 00:00:00 Reading config file: /usr/local/share/snpeff-5.1-2/snpEff.config java.lang.RuntimeException: Property: 'pmeandrina1.genome' not found at org.snpeff.interval.Genome.(Genome.java:104) at org.snpeff.snpEffect.Config.readGenomeConfig(Config.java:784) at org.snpeff.snpEffect.Config.readConfig(Config.java:751) at org.snpeff.snpEffect.Config.init(Config.java:529) at org.snpeff.snpEffect.Config. (Config.java:116) at org.snpeff.SnpEff.loadConfig(SnpEff.java:429) at org.snpeff.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:889) at org.snpeff.snpEffect.commandLine.SnpEffCmdEff.run(SnpEffCmdEff.java:875) at org.snpeff.SnpEff.run(SnpEff.java:1141) at org.snpeff.SnpEff.main(SnpEff.java:160) 00:00:00 Logging 00:00:01 Done. Work dir: /mmfs1/gscratch/scrubbed/strigg/analyses/20250721_rnavar/work/36/1eae8876f1fa46934db2903e21630b Container: /gscratch/scrubbed/srlab/.apptainer/depot.galaxyproject.org-singularity-snpeff-5.1--hdfd78af_2.img Tip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line
nextflow run nf-core/rnavar -c /gscratch/srlab/strigg/bin/uw_hyak_srlab.config -resume --input samplesheet.csv --genome null --igenomes_ignore --fasta /gscratch/srlab/strigg/GENOMES/Pocillopora_meandrina_HIv1.assembly.fasta --gtf /gscratch/srlab/strigg/GENOMES/Pocillopora_meandrina_HIv1.genes-validated.gtf --snpeff_cache /gscratch/srlab/strigg/bin/snpEff/data --snpeff_db pmeandrina1 --tools snpeff --skip_baserecalibration true --outdir result
6829325bfeff043a2d4dd1772e7aad18
c0b4c304-c027-4731-af72-9c6ca585d1ad
https://github.com/nf-core/rnavar
, revision master
(commit hash 6a5ca7896f12ffc3b8a8c97c21cd0abcd7bf2e36
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