id: "nf-core-rnavar-methods-description"
description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication."
section_name: "nf-core/rnavar Methods Description"
section_href: "https://github.com/nf-core/rnavar"
plot_type: "html"
data: |
  <h4>Methods</h4>
  <p>Data was processed using nf-core/rnavar v1.1.0 (doi: <a href='https://doi.org/10.5281/zenodo.6669636'>10.5281/zenodo.6669636</a>) of the nf-core collection of workflows (<a href="https://doi.org/10.1038/s41587-020-0439-x">Ewels <em>et al.</em>, 2020</a>), utilising reproducible software environments from the Bioconda (<a href="https://doi.org/10.1038/s41592-018-0046-7">Grüning <em>et al.</em>, 2018</a>) and Biocontainers (<a href="https://doi.org/10.1093/bioinformatics/btx192">da Veiga Leprevost <em>et al.</em>, 2017</a>) projects.</p>
  <p>The pipeline was executed with Nextflow v24.10.6 (<a href="https://doi.org/10.1038/nbt.3820">Di Tommaso <em>et al.</em>, 2017</a>) with the following command:</p>
  <pre><code>nextflow run nf-core/rnavar -c /gscratch/srlab/strigg/bin/uw_hyak_srlab.config -resume --input samplesheet.csv --genome null --igenomes_ignore --fasta /gscratch/srlab/strigg/GENOMES/Porites_evermanni_v1.fa --gtf /gscratch/srlab/strigg/GENOMES/Porites_evermanni_validated.gtf --skip_variantannotation --skip_baserecalibration true --outdir result</code></pre>
  <p></p>
  <h4>References</h4>
  <ul>
    <li>Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: <a href="https://doi.org/10.1038/nbt.3820">10.1038/nbt.3820</a></li>
    <li>Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: <a href="https://doi.org/10.1038/s41587-020-0439-x">10.1038/s41587-020-0439-x</a></li>
    <li>Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: <a href="https://doi.org/10.1038/s41592-018-0046-7">10.1038/s41592-018-0046-7</a></li>
    <li>da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: <a href="https://doi.org/10.1093/bioinformatics/btx192">10.1093/bioinformatics/btx192</a></li>
    
  </ul>
  <div class="alert alert-info">
    <h5>Notes:</h5>
    <ul>
      
      <li>The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!</li>
      <li>You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.</li>
    </ul>
  </div>