+ bcftools --version
bcftools 1.22
Using htslib 1.22.1
Copyright (C) 2025 Genome Research Ltd.
License GPLv3+: GNU GPL version 3 or later <http://gnu.org/licenses/gpl.html>
This is free software: you are free to change and redistribute it.
There is NO WARRANTY, to the extent permitted by law.
+ echo 'Hard filtering variants'
Hard filtering variants
+ bcftools filter -e 'QUAL < 30.0 || FMT/DP < 10 || FMT/GQ < 20 || FMT/DP > 150 || FMT/AF1 < 0.3' --soft-filter FAIL -Oz -o Peve.biscuit.merged-filtered.vcf.gz Peve.biscuit.merged.vcf.gz
+ bcftools index merged-filtered.vcf.gz
index: failed to open merged-filtered.vcf.gz