Bismark report for: /Volumes/web/seashell/bu-serine-wd/18-04-07/zr2096_8_s1_R1.fastq.gz and /Volumes/web/seashell/bu-serine-wd/18-04-07/zr2096_8_s1_R2.fastq.gz (version: v0.20.0) Bismark was run with Bowtie 2 against the bisulfite genome of /Volumes/web/metacarcinus/Cvirginica/GENOMES/v300/Bismark_genome/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 150 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 100000 Number of paired-end alignments with a unique best hit: 14354 Mapping efficiency: 14.4% Sequence pairs with no alignments under any condition: 81679 Sequence pairs did not map uniquely: 3967 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3601 ((converted) top strand) GA/CT/CT: 3584 (complementary to (converted) top strand) GA/CT/GA: 3585 (complementary to (converted) bottom strand) CT/GA/GA: 3584 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 492720 Total methylated C's in CpG context: 34212 Total methylated C's in CHG context: 3523 Total methylated C's in CHH context: 19647 Total methylated C's in Unknown context: 1841 Total unmethylated C's in CpG context: 21124 Total unmethylated C's in CHG context: 99079 Total unmethylated C's in CHH context: 315135 Total unmethylated C's in Unknown context: 7158 C methylated in CpG context: 61.8% C methylated in CHG context: 3.4% C methylated in CHH context: 5.9% C methylated in unknown context (CN or CHN): 20.5% Bismark completed in 0d 0h 4m 6s