Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_9_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_9_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 32636231
Paired-end alignments with a unique best hit 10389383
Pairs without alignments under any condition 19378195
Pairs that did not map uniquely 2868653
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 393776621
Methylated C's in CpG context 40612390
Methylated C's in CHG context 2982484
Methylated C's in CHH context 10534650
Methylated C's in Unknown context 1349020
Unmethylated C's in CpG context 13936979
Unmethylated C's in CHG context 84223643
Unmethylated C's in CHH context 241486475
Unmethylated C's in Unknown context 5462880
Percentage methylation (CpG context) 74.5%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 4.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2817892 original top strand
CTOT 2366113 complementary to original top strand
CTOB 2380610 complementary to original bottom strand
OB 2824766 original bottom strand

Deduplication

Alignments analysed 10389381
Unique alignments 9180203
Duplicates removed 1209178
Duplicated alignments were found at 999307 different positions

Cytosine Methylation after Extraction

Total C's analysed 247569836
Methylated C's in CpG context 24620940
Methylated C's in CHG context 2094757
Methylated C's in CHH context 7736902
Unmethylated C's in CpG context 9054979
Unmethylated C's in CHG context 52006634
Unmethylated C's in CHH context 152055624
Percentage methylation (CpG context) 73.1%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 4.8%

M-Bias Plot