Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_8_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_8_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 29761837
Paired-end alignments with a unique best hit 9031924
Pairs without alignments under any condition 18352667
Pairs that did not map uniquely 2377246
Genomic sequence context not extractable (edges of chromosomes) 5

Cytosine Methylation

Total C's analysed 319685131
Methylated C's in CpG context 25081844
Methylated C's in CHG context 2220090
Methylated C's in CHH context 9298556
Methylated C's in Unknown context 1029157
Unmethylated C's in CpG context 13629344
Unmethylated C's in CHG context 67667514
Unmethylated C's in CHH context 201787783
Unmethylated C's in Unknown context 4326319
Percentage methylation (CpG context) 64.8%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 4.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2310227 original top strand
CTOT 2208932 complementary to original top strand
CTOB 2211243 complementary to original bottom strand
OB 2301517 original bottom strand

Deduplication

Alignments analysed 9031919
Unique alignments 8727936
Duplicates removed 303983
Duplicated alignments were found at 261034 different positions

Cytosine Methylation after Extraction

Total C's analysed 223405198
Methylated C's in CpG context 17003347
Methylated C's in CHG context 1714400
Methylated C's in CHH context 7367274
Unmethylated C's in CpG context 9662409
Unmethylated C's in CHG context 46581820
Unmethylated C's in CHH context 141075948
Percentage methylation (CpG context) 63.8%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 5.0%

M-Bias Plot