Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_7_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_7_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 29534746
Paired-end alignments with a unique best hit 10263536
Pairs without alignments under any condition 16560814
Pairs that did not map uniquely 2710396
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 369380706
Methylated C's in CpG context 32900574
Methylated C's in CHG context 2617073
Methylated C's in CHH context 9978987
Methylated C's in Unknown context 1167787
Unmethylated C's in CpG context 13974627
Unmethylated C's in CHG context 78100593
Unmethylated C's in CHH context 231808852
Unmethylated C's in Unknown context 4963777
Percentage methylation (CpG context) 70.2%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 4.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2605817 original top strand
CTOT 2517619 complementary to original top strand
CTOB 2532159 complementary to original bottom strand
OB 2607940 original bottom strand

Deduplication

Alignments analysed 10263535
Unique alignments 9955889
Duplicates removed 307646
Duplicated alignments were found at 262839 different positions

Cytosine Methylation after Extraction

Total C's analysed 259380298
Methylated C's in CpG context 22460519
Methylated C's in CHG context 2034052
Methylated C's in CHH context 7897415
Unmethylated C's in CpG context 10024937
Unmethylated C's in CHG context 54122929
Unmethylated C's in CHH context 162840446
Percentage methylation (CpG context) 69.1%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 4.6%

M-Bias Plot