Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_6_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_6_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 24237290
Paired-end alignments with a unique best hit 7735314
Pairs without alignments under any condition 14340992
Pairs that did not map uniquely 2160984
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 275523644
Methylated C's in CpG context 25657852
Methylated C's in CHG context 1838651
Methylated C's in CHH context 7806415
Methylated C's in Unknown context 911519
Unmethylated C's in CpG context 10345208
Unmethylated C's in CHG context 58273355
Unmethylated C's in CHH context 171602163
Unmethylated C's in Unknown context 3902421
Percentage methylation (CpG context) 71.3%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 4.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2038524 original top strand
CTOT 1822720 complementary to original top strand
CTOB 1834457 complementary to original bottom strand
OB 2039611 original bottom strand

Deduplication

Alignments analysed 7735312
Unique alignments 7550283
Duplicates removed 185029
Duplicated alignments were found at 153083 different positions

Cytosine Methylation after Extraction

Total C's analysed 190864683
Methylated C's in CpG context 17248355
Methylated C's in CHG context 1442731
Methylated C's in CHH context 6328119
Unmethylated C's in CpG context 7336625
Unmethylated C's in CHG context 39733116
Unmethylated C's in CHH context 118775737
Percentage methylation (CpG context) 70.2%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 5.1%

M-Bias Plot