Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_5_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_5_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 31778715
Paired-end alignments with a unique best hit 10309461
Pairs without alignments under any condition 18728694
Pairs that did not map uniquely 2740560
Genomic sequence context not extractable (edges of chromosomes) 5

Cytosine Methylation

Total C's analysed 370441524
Methylated C's in CpG context 30590802
Methylated C's in CHG context 2478038
Methylated C's in CHH context 10020162
Methylated C's in Unknown context 1213895
Unmethylated C's in CpG context 15985714
Unmethylated C's in CHG context 76625684
Unmethylated C's in CHH context 234741124
Unmethylated C's in Unknown context 5096608
Percentage methylation (CpG context) 65.7%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 4.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2677514 original top strand
CTOT 2476905 complementary to original top strand
CTOB 2486673 complementary to original bottom strand
OB 2668364 original bottom strand

Deduplication

Alignments analysed 10309456
Unique alignments 9873285
Duplicates removed 436171
Duplicated alignments were found at 368608 different positions

Cytosine Methylation after Extraction

Total C's analysed 252492402
Methylated C's in CpG context 20157186
Methylated C's in CHG context 1871699
Methylated C's in CHH context 7828745
Unmethylated C's in CpG context 11121885
Unmethylated C's in CHG context 51406712
Unmethylated C's in CHH context 160106175
Percentage methylation (CpG context) 64.4%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 4.7%

M-Bias Plot