Bismark report for: /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_4_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_4_s1_R2.fastq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/strigg/data/Cvirg/Bismark_genome/ with the specified options: -q --score-min L,0,-1.2 --ignore-quals --no-mixed --no-discordant --dovetail --minins 100 --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 24341968 Number of paired-end alignments with a unique best hit: 7587323 Mapping efficiency: 31.2% Sequence pairs with no alignments under any condition: 14662643 Sequence pairs did not map uniquely: 2092002 Sequence pairs which were discarded because genomic sequence could not be extracted: 1 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1946078 ((converted) top strand) GA/CT/CT: 1835678 (complementary to (converted) top strand) GA/CT/GA: 1856529 (complementary to (converted) bottom strand) CT/GA/GA: 1949037 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 268056140 Total methylated C's in CpG context: 21938186 Total methylated C's in CHG context: 1744867 Total methylated C's in CHH context: 7735389 Total methylated C's in Unknown context: 834133 Total unmethylated C's in CpG context: 12374431 Total unmethylated C's in CHG context: 56852641 Total unmethylated C's in CHH context: 167410626 Total unmethylated C's in Unknown context: 3707185 C methylated in CpG context: 63.9% C methylated in CHG context: 3.0% C methylated in CHH context: 4.4% C methylated in unknown context (CN or CHN): 18.4% Bismark completed in 0d 4h 40m 7s