Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_4_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_4_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 24341968
Paired-end alignments with a unique best hit 7587323
Pairs without alignments under any condition 14662643
Pairs that did not map uniquely 2092002
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 268056140
Methylated C's in CpG context 21938186
Methylated C's in CHG context 1744867
Methylated C's in CHH context 7735389
Methylated C's in Unknown context 834133
Unmethylated C's in CpG context 12374431
Unmethylated C's in CHG context 56852641
Unmethylated C's in CHH context 167410626
Unmethylated C's in Unknown context 3707185
Percentage methylation (CpG context) 63.9%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 4.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1946078 original top strand
CTOT 1835678 complementary to original top strand
CTOB 1856529 complementary to original bottom strand
OB 1949037 original bottom strand

Deduplication

Alignments analysed 7587322
Unique alignments 7423370
Duplicates removed 163952
Duplicated alignments were found at 136766 different positions

Cytosine Methylation after Extraction

Total C's analysed 186384892
Methylated C's in CpG context 14778915
Methylated C's in CHG context 1374799
Methylated C's in CHH context 6316301
Unmethylated C's in CpG context 8756708
Unmethylated C's in CHG context 38923593
Unmethylated C's in CHH context 116234576
Percentage methylation (CpG context) 62.8%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 5.2%

M-Bias Plot