Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_3_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_3_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 29892002
Paired-end alignments with a unique best hit 10170153
Pairs without alignments under any condition 16789874
Pairs that did not map uniquely 2931975
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 380186993
Methylated C's in CpG context 41316176
Methylated C's in CHG context 2588230
Methylated C's in CHH context 9338662
Methylated C's in Unknown context 1160192
Unmethylated C's in CpG context 13734426
Unmethylated C's in CHG context 82498352
Unmethylated C's in CHH context 230711147
Unmethylated C's in Unknown context 4951346
Percentage methylation (CpG context) 75.1%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 3.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2554767 original top strand
CTOT 2514160 complementary to original top strand
CTOB 2537491 complementary to original bottom strand
OB 2563734 original bottom strand

Deduplication

Alignments analysed 10170152
Unique alignments 9734305
Duplicates removed 435847
Duplicated alignments were found at 329738 different positions

Cytosine Methylation after Extraction

Total C's analysed 262062166
Methylated C's in CpG context 27665110
Methylated C's in CHG context 2000329
Methylated C's in CHH context 7413378
Unmethylated C's in CpG context 9692932
Unmethylated C's in CHG context 56032292
Unmethylated C's in CHH context 159258125
Percentage methylation (CpG context) 74.1%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 4.4%

M-Bias Plot