Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_2_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_2_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 30798582
Paired-end alignments with a unique best hit 7879875
Pairs without alignments under any condition 20682061
Pairs that did not map uniquely 2236646
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 282047529
Methylated C's in CpG context 25969563
Methylated C's in CHG context 1938729
Methylated C's in CHH context 9023855
Methylated C's in Unknown context 934954
Unmethylated C's in CpG context 11825530
Unmethylated C's in CHG context 61284539
Unmethylated C's in CHH context 172005313
Unmethylated C's in Unknown context 3930167
Percentage methylation (CpG context) 68.7%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 5.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1988714 original top strand
CTOT 1934508 complementary to original top strand
CTOB 1961754 complementary to original bottom strand
OB 1994898 original bottom strand

Deduplication

Alignments analysed 7879874
Unique alignments 7582898
Duplicates removed 296976
Duplicated alignments were found at 239515 different positions

Cytosine Methylation after Extraction

Total C's analysed 190616346
Methylated C's in CpG context 16881804
Methylated C's in CHG context 1484733
Methylated C's in CHH context 7213704
Unmethylated C's in CpG context 8165931
Unmethylated C's in CHG context 40637178
Unmethylated C's in CHH context 116232996
Percentage methylation (CpG context) 67.4%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 5.8%

M-Bias Plot