Bismark

Bismark Processing Report

/gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_1_s1_R1.fastq.gz and /gscratch/srlab/strigg/data/Cvirg/FASTQS/18-04-07/zr2096_1_s1_R2.fastq.gz

Data processed at 03:44 on 2018-11-07


Alignment Stats

Sequence pairs analysed in total 28982766
Paired-end alignments with a unique best hit 4521752
Pairs without alignments under any condition 23239578
Pairs that did not map uniquely 1221436
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 163653583
Methylated C's in CpG context 14475033
Methylated C's in CHG context 1101550
Methylated C's in CHH context 4900521
Methylated C's in Unknown context 581418
Unmethylated C's in CpG context 6611427
Unmethylated C's in CHG context 33757094
Unmethylated C's in CHH context 102807958
Unmethylated C's in Unknown context 2347522
Percentage methylation (CpG context) 68.6%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 4.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1135164 original top strand
CTOT 1123470 complementary to original top strand
CTOB 1130861 complementary to original bottom strand
OB 1132256 original bottom strand

Deduplication

Alignments analysed 4521751
Unique alignments 4407890
Duplicates removed 113861
Duplicated alignments were found at 87450 different positions

Cytosine Methylation after Extraction

Total C's analysed 117187042
Methylated C's in CpG context 10156041
Methylated C's in CHG context 867748
Methylated C's in CHH context 3830928
Unmethylated C's in CpG context 4825412
Unmethylated C's in CHG context 23903571
Unmethylated C's in CHH context 73603342
Percentage methylation (CpG context) 67.8%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 4.9%

M-Bias Plot