FastQCFastQC Report
Wed 30 Apr 2025
2C3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13949991
Total Bases351.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA196649414.096740277466846No Hit
CTCGGGCTGAGACTTGAAGCG8910596.387523834244768No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5324893.817127910691842No Hit
GTTGGATCCGGCCCGGACTGGCC4956313.5529126864669665No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA4115232.949987566300222No Hit
CTCGGGCTGAGACTTGAAGCGGTT3634232.605184476463103No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC2630531.885685804385107No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT1635671.172524053958171No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT1622611.1631620407497036No Hit
CTCGGGCTGAGACTTGAAGC1551711.112337635199908No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1510641.0828967559907385No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC1316330.9436063435453113No Hit
CGGACTGGCCGTGGTCGAGGT960800.6887459640654965No Hit
CTCGGGCTGAGACTTGAAGCGG743830.5332118135416718No Hit
TTCCGAGGTCGCGGTCGGACCG730920.5239573272842972No Hit
CAGCTGTGACCCTCGGGTC729010.5225881507737173No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC722680.5180505134376072No Hit
CAGCTGTGACCCTCGGGT722030.5175845633162057No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC646220.46324044223397703No Hit
CGGACTGGCCGTGGTCGAGGC608010.4358497435589743No Hit
CTCGGGCTGAGACTTGAAGCA572860.41065259468626186No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA569910.4085378979814395No Hit
CTCGGGCTGAGACTTGAAGCGGTTG541820.3884016842734881No Hit
GTTGGATCCGGCCCGGACTGGC541300.38802892417636686No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC529520.3795844742838902No Hit
GGAAGGAATCGGCCGTGGATTGGCC488810.3504016597573432No Hit
CGAGTGTGCTCTTAACTG449630.3223156201319413No Hit
AGACTTGAAGCGGTTGGAT429830.30812206258771063No Hit
GCACTGTGGTTCAGTGGTAGAATTCT429510.30789267175871293No Hit
CTCGGGCTGAGACTTGAAGCGGT405580.29073853882773115No Hit
CGGACTGGCCGTGGTCGAGG388190.27827258096438917No Hit
GAGACTTGAAGCGGTTGGAT380730.27292490726338103No Hit
TGAAAATCTTTGCTCTGAAGTGGA379300.2718998169962977No Hit
CGTGGTGGACTGCTTGCAGTGCT373600.26781379285477674No Hit
TTCTGCTGAACTACTTTGAGAACGAGT372620.2671112834409714No Hit
GTTGGATCCGGCCCGGACTGGCT344800.24716861824498668No Hit
GACCAAGATAGTCGAACTCGACCCTGTC334380.23969907937575013No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA324080.23231556206738774No Hit
CTTTCCGAGGTCGCGGTC323010.23154853648292675No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG316430.22683168756166225No Hit
TGAAAATCTTTGCTCTGAAGTGGAT314950.22577075497754803No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT307850.22068114595916224No Hit
TCTCGGGCTGAGACTTGAAGCG291550.20899655060709357No Hit
CGGACTGGCCGTGGTCGAGGTGA290400.2081721773153832No Hit
CGACTGCTGCCGGTCGTTGGGGCG288350.2067026423171169No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG266780.1912402667499929No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCG253290.18157000961505995No Hit
GAGACTTGAAGCGGTTGGA242270.1736703629414528No Hit
TACGTTGGCTTTGTAGATTACCCTGGTGGT238630.17106104226160432No Hit
AGACTTGAAGCGGTTGGATCCGGCCC237500.17025100589670633No Hit
ACCCGTAGATCCGAACTTGTGG235360.16871695472778442No Hit
CGACTGCTGCCGGTCGTTGGGGC232580.16672412190086716No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA215650.15458791335420935No Hit
TGGGGGTAGTCGTTCTATGT207330.14862375180027No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA205080.14701084753388013No Hit
GAATCGGCCGTGGATTGGC202820.1453907748040841No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG194580.13948396095739415No Hit
CGGACTGGCCGTGGTCGAGGTG192470.1379714151786908No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC189640.13594274003474266No Hit
CACTACCGATAGCCAACTCGAACGTTTCCG189260.13567033842530793No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA185260.1328029530628371No Hit
TTGGATCCGGCCCGGACTGGCC180270.12922588982315472No Hit
GTTGGATCCGGCCCGGACT179600.12874560277494088No Hit
TTCCGAGGTCGCGGTCGGACC177650.12734775241073631No Hit
TGAAAATCTTTGCTCTGAAGTGG174740.1252617295595388No Hit
TTCCGAGGTCGCGGTCGGACCGGGA173590.12443735626782843No Hit
GTGTGTGATGACTGCATAGTTCAACTGAACA169300.12136208546657844No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA167700.12021513132159009No Hit
GAGACCGAGTGTGCTCTTAACTG166310.11921871490813148No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT160140.11479577298652022No Hit
GACTTGAAGCGGTTGGATCCGGCCC159840.1145807190843349No Hit
CGGACTGGCCGTGGTCGAGGTGAG156750.11236566389182616No Hit
GGATCCGGCCCGGACTGGCC154760.11093913967399692No Hit
GACTTGAAGCGGTTGGAT153000.10967749011450975No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC148490.10644451311832388No Hit
GTTGGATCCGGCCCGGACTGGCA146740.10519003202224288No Hit
TCTCGGGCTGAGACTTGAAGCGGTT146650.10512551585158728No Hit
AGACTTGAAGCGGTTGGATCCGGCC143200.10265239597645617No Hit
GGATCCGGCCCGGACTGGC141890.10171332727024698No Hit
CCGAGGTCGCGGTCGGACCGGGAAG141060.10111834480753427No Hit
CTCGGGCTGAGACTTGAAGCGGTC139500.10000006451617066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)