FastQCFastQC Report
Wed 30 Apr 2025
2A2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9913571
Total Bases258.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA301390030.401759366024617No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC8310308.38275128104696No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1554471.5680222595873878No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1378981.3910022937244308No Hit
CAGCTGTGACCCTCGGGTC1249931.2608272034365822No Hit
CGATCAAGATAGTCGAACTCGACCCTGTC1048151.057288034755589No Hit
CGGACTGGCCGTGGTCGAGGC797830.8047856821724483No Hit
TGAAAATCTTTGCTCTGAAGTGGA624550.6299949836441379No Hit
CTCGGGCTGAGACTTGAAGCG519510.5240392185621104No Hit
TGAAAATCTTTGCTCTGAAGTGGAT483570.48778588462220124No Hit
CGTGGTGGACTGCTTGCAGTGCT435800.43959941377330125No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC415560.4191829563736417No Hit
GTTGGATCCGGCCCGGACTGGCC414370.41798258165498586No Hit
TTACCGTGACCGATCCCGT388140.39152390193200814No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT386580.3899503014604929No Hit
TTGTGTAACTCCCTAAGGAAGG384530.38788242904600173No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT362170.36532748895428296No Hit
ACCCGTAGATCCGAACTTGTGG345870.34888538146345044No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA339570.34253045648233116No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTC337430.34037179942525253No Hit
ACCCGTAGATCCGAACTTGT334680.3375978242350814No Hit
CACCCCTCATCCGCTACTTTGACCTCATT292790.2953426167018928No Hit
TGAAAATCTTTGCTCTGAAGTGG271530.27389726668624254No Hit
CGATCAAGATAGTCGAACTCGACCCTGTCA256750.2589884109368864No Hit
TCAAGTCTAGGCTGGTTAGTTT246050.2481951256514933No Hit
CGACTGCTGCCGGTCGTTGGGGCG242670.24478565796321022No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC241630.24373659098220007No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTCA225710.22767779642673663No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC210250.21208301226672005No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA209030.210852376000535No Hit
CTCGGGCTGAGACTTGAAGC205930.2077253494225239No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC205640.20743282112974226No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTT197120.1988385416314666No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT184310.185916860836524No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA169610.1710887025472456No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG162680.16409828506801433No Hit
CGAGTGTGCTCTTAACTG158690.16007349924663877No Hit
GTGTGTGATGACTGCATAGTTCAACTGAACA156060.1574205702465842No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC148620.1499157064593576No Hit
TGAAAATCTTTGCTCTGAAGTGGGA147100.14838245471788117No Hit
TCGTCTGATTGGATACCCACGTACTTGATC146470.14774696221976924No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA146120.1473939108319293No Hit
CCGATCAAGATAGTCGAACTCGACCCTGT145100.14636501821593853No Hit
TGAAAATCTTTGCTCTGAAGTGGAAA142020.14325816600294686No Hit
TGAAAATCCTTGCTCTGAAGTGGAA129070.13019526465286826No Hit
TGACTTAAGTACGGCTGCAACAGAAGC121320.12237769820784053No Hit
TGAAAATCTTTGCTCTGAAGTGGAG119260.12029973861083963No Hit
CGATCAAGATAGTCGAACTCGACCCTGTT117900.11892788178951863No Hit
CGACTGCTGCCGGTCGTTGGGGC112990.11397507517724945No Hit
TGAAAATCTTTGCTCTGAAGTGGTA110630.11159450010495714No Hit
CTTTCCGAGGTCGCGGTC109850.11080769986919951No Hit
TGAAAATCTTTGCTCTGAGGTGGAA107270.1082052067816935No Hit
TCGAAGACTCTAAACGGCGGAAGCCTATT104690.10560271369418749No Hit
CGAAAATCTTTGCTCTGAAGTGGAA104460.10537070849646409No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT102740.10363571310479341No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCC99290.10015563513894236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)