FastQCFastQC Report
Wed 30 Apr 2025
1H9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8040284
Total Bases187.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG86130310.712345484313738No Hit
CGTGGTGGACTGCTTGCAGTGCT8014929.968453850635127No Hit
CTCGGGCTGAGACTTGAAGCGGTT6082137.564571102214797No Hit
CTCGGGCTGAGACTTGAAGC2641173.2849212789000983No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA2534543.152301585366885No Hit
CAGCTGTGACCCTCGGGTC2211352.750338172134218No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT1723832.143991431148452No Hit
TGAAAATCTTTGCTCTGAAGTGGAA1076691.3391193644403605No Hit
GTTGGATCCGGCCCGGACTGGCC897391.1161172913792599No Hit
CAGCTGTGACCCTCGGGT841541.0466545709081918No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC829521.0317048502266835No Hit
CGGACTGGCCGTGGTCGAGGC822091.0224638831165664No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA758610.9435114481030771No Hit
CTTTCCGAGGTCGCGGTC616600.7668883337951744No Hit
TCTCGGGCTGAGACTTGAAGCGGTT559330.6956595065547436No Hit
TGAAAATCTTTGCTCTGAGGTGGAA538360.6695783382776032No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC509210.6333234000192033No Hit
GAGTGTGCTCTTAACTGA464190.5773303530074311No Hit
CGTGGTGGACTGCTTGCAGTGCC436150.542455962998322No Hit
TCTCGGGCTGAGACTTGAAGCG374920.46630193659825947No Hit
GGAAGGAATCGGCCGTGGATTGGCC367770.4574092158933689No Hit
CTCGGGCTGAGACTTGAAGCGGTTG364140.4528944499970399No Hit
GAACTGGGCGTCGGAGGTGCCGTGGAAGGTA353170.4392506533351309No Hit
GAGTGTGCTCTTAACTG350440.43585525088417276No Hit
CTCGGGCTGAGACTTGAAGCA340750.4238034377890134No Hit
CGTGGTGGACTGCTTGCAGT335860.41772156306916525No Hit
CTCGGGCTGAGACTTGAAGCGG324440.40351808468457084No Hit
GAGACTTGAAGCGGTTGGAT319340.3971750251608027No Hit
GAGTGTGCTCTTAACT307570.3825362387696753No Hit
GATCCGGCCCGGACTGGCC292810.3641786782655936No Hit
CGAGTGTGCTCTTAACTG276720.34416694733668607No Hit
CTTTCCGAGGTCGCGGTCGGA274590.3415177871826418No Hit
GAGACTTGAAGCGGTTGGA270110.3359458446990181No Hit
GCCATGGGGGACAAGAGC265650.33039877695862485No Hit
GCCGGGAAGTTGTTCAAACTTGATCA260370.3238318447457826No Hit
GGATCCGGCCCGGACTGGCC244810.3044792945124824No Hit
GAGACCGAGTGTGCTCTTAACTG237510.2954000132333634No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT234930.2921911713566337No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA234330.29144492905971975No Hit
CGTGGTGGACTGCTTGCA228900.2846914362726491No Hit
CGTGGTGGACTGCTTGCAGTGCTC212560.2643687710533608No Hit
CGGACTGGCCGTGGTCGAGGCGAGG206110.25634666636153647No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT203060.2525532680188909No Hit
CTCGGGCTGAGACTTGAAGCGGT202660.2520557731542816No Hit
GACCGTGGTGGACTGCTTGCAGTGCT201720.2508866602224498No Hit
CGTAGTTGGATTTCGGGATGGCCCG195310.24291430501708647No Hit
GGACTGGCCGTGGTCGAGGC189010.23507876089949062No Hit
CTTTCCGAGGTCGCGGTCGGACCG185850.23114855146907748No Hit
CTCGGGCTGAGACTTGAAGCGGTC172460.21449491087628247No Hit
GAGACCGAGTGTGCTCTTAACTGA162060.2015600443964417No Hit
TTGGATCCGGCCCGGACTGGCC154940.19270463580639688No Hit
GAGACCGAGTGTGCTCTTAACT148740.18499346540495337No Hit
GGAAGGAATCGGCCGTGGATTGGCCCA135590.1686383217309239No Hit
CTCCTGACTGGCGCCGACA128680.16004409794479896No Hit
CTCGTTGTGTGTGTGTCTCTCTGTCT128340.1596212273098811No Hit
GAGGTGCCGTGGAAGGTAGGTCT124660.1550442745554759No Hit
AACAGTAGTGGGATAGCA123980.15419853328564015No Hit
GAGACCGAGTGTGCTCTTAACTGAG123020.15300454561057794No Hit
CGGTTAGGCGAACAAGAATAGAGGCC121190.15072850660499057No Hit
AGTGTGCTCTTAACT120650.15005688853776808No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA114830.14281833825770335No Hit
GAGACCGAGTGTGCTCTTAACTGAGTGT112280.13964680849581929No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC111960.1392488126041319No Hit
TCCCTTTCCGAGGTCGCGGTC110770.13776876538191934No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC107230.1333659358301274No Hit
AGTGTGCTCTTAACTG106380.13230875924283272No Hit
TTAGTGAGGCCTCCTGACTGGCGCC104840.13039340401408708No Hit
CTTTCCGAGGTCGCG99080.12322947796371372No Hit
CTTAACTGAGTGTGCT97480.12123949850527668No Hit
CGTGGTGGACTGCTTGCAGTGCA96340.11982163814114029No Hit
CCTGACTGGCGCCGACACTCTGTCTCGTGCA94350.11734660118970922No Hit
TCTCGGGCTGAGACTTGAAGC92690.11528199750158079No Hit
CGCCGAGACCGAGTGTGCT90580.11265771209076694No Hit
CCCTTTCCGAGGTCGCGGTC90070.11202340613839014No Hit
GTCTAATGACTCGGATGGAGCTT87780.1091752480385021No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG87370.10866531580227762No Hit
GGAAGGAATCGGCCGTGGATT87140.1083792562551273No Hit
CGGGCTGAGACTTGAAGCGGTT86860.10803100984990083No Hit
CTGTTCTTCCTCGCCGAGACCGAGTGTGCT85790.10670021108707105No Hit
GGAAGGAATCGGCCGTGGAT84480.10507091540547572No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC83620.10400130144656583No Hit
GAGACCGAGTGTGCTCTTAACTGAGTGTG83400.10372767927103073No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA83000.10323018440642145No Hit
CTTTCCGAGGTCGCGGTCGGACC82910.10311824806188438No Hit
GTGAGGCCTCCTGACTGGCGCC81770.10170038769774799No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC81670.10157601398159566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)