FastQCFastQC Report
Wed 30 Apr 2025
1H5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9255488
Total Bases216 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG6594637.125102425717585No Hit
CGTGGTGGACTGCTTGCAGTGCT4585004.9538176701217695No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA3566463.853346252515265No Hit
CGGACTGGCCGTGGTCGAGGC3038833.283273664230346No Hit
CAGCTGTGACCCTCGGGTC2597392.806324204623246No Hit
CTCGGGCTGAGACTTGAAGCGGTT2148152.3209473125566147No Hit
CTCGGGCTGAGACTTGAAGC1650101.78283414121438No Hit
CAGCTGTGACCCTCGGGT1608811.7382227711818112No Hit
GTTGGATCCGGCCCGGACTGGCC1599731.72841237544687No Hit
CTCGGGCTGAGACTTGAAGCA1154221.247065524799989No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT1023801.1061545323163942No Hit
CGACTGCTGCCGGTCGTTGGGGC809670.8747999025010891No Hit
GAATCGGCCGTGGATTGGC635720.6868573542529578No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT593800.641565306983273No Hit
CTTTCCGAGGTCGCGGTC587210.6344452069950282No Hit
GGACTGGCCGTGGTCGAGGC586860.6340670529744082No Hit
CGTGGTGGACTGCTTGCAGTGCC580680.6273899334103183No Hit
GGAAGGAATCGGCCGTGGATTGGCC558490.6034149685030115No Hit
CGGACTGGCCGTGGTCGAGGT519900.561720786629511No Hit
CGTGGTGGACTGCTTGCAGTGCTC509340.5503113396073767No Hit
GAGACTTGAAGCGGTTGGA478330.5168068933804463No Hit
CGAGTGTGCTCTTAACTG471080.5089737029533181No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC388070.41928637366284743No Hit
GTTGGATCCGGCCCGGACTGGCT346960.3748694828408832No Hit
GAGACTTGAAGCGGTTGGAT325070.35121864995125057No Hit
CGTGGTGGACTGCTTGCAGTGCA312540.33768073601305515No Hit
CGACTGCTGCCGGTCGTTGGGGCG296470.32031806426630344No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT295230.31897831859324977No Hit
CTCGGGCTGAGACTTGAAGCGG293130.3167093944695299No Hit
TGAAAATCTTTGCTCTGAAGTGGAA290990.3143972527434534No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC279230.301691277650622No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA276080.29828789146504214No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC275370.29752077902321306No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC244430.2640919636004066No Hit
AGACTTGAAGCGGTTGGAT243510.2630979587462055No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC236730.255772575146767No Hit
CTCGGGCTGAGACTTGAAG227990.24632952903185656No Hit
CTCGGGCTGAGACTTGAAGCGGTTG220240.23795611857527124No Hit
CTCGGGCTGAGACTTGAAGT211160.22814572284033No Hit
GAGTGTGCTCTTAACTG210550.22748665440439228No Hit
TCTCGGGCTGAGACTTGAAGCG202760.21907002634545042No Hit
CTCGGGCTGAGACTTGAAGCGGTC195490.2112152271171439No Hit
GAGTGTGCTCTTAACT192100.20755253531742462No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT187670.2027661858564346No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCG186880.20191263820989233No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA179170.1935824453556636No Hit
CAGCTGTGACCCTCGGGC177950.1922643084837882No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG176190.19036273398009915No Hit
GTTGGATCCGGCCCGGACTGGCA172780.18667843337920162No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC172600.18648395416859703No Hit
CTCGGGCTGAGACTTGAAGCGGT171570.1853711009079154No Hit
CAGCTGTGACCCTCGGGTT170410.18411779043957488No Hit
CGACTGCTGCCGGTCGTTGGGGT167800.18129784188580872No Hit
AGACTTGAAGCGGTTGGA160250.17314051944100622No Hit
GACCGTGGTGGACTGCTTGCAGTGCT156690.16929415283127158No Hit
GAGTGTGCTCTTAACTGA156660.16926173962950414No Hit
CGTGGTGGACTGCTTGCAGTGCG155590.1681056687664659No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGT148330.16026167393874854No Hit
AGTGTGCTCTTAACT148300.16022926073698113No Hit
CGTGGTGGACTGCTTGCAGT145170.1568474833525796No Hit
GACTGCTGCCGGTCGTTGGGGC145150.1568258745514013No Hit
CTCGGGCTGAGACTTGAAGCT136670.14766374285180855No Hit
GAGACCGAGTGTGCTCTTAACTG132050.1426721097796248No Hit
TGGGGGTAGTCGTTCTATGT129310.1397117040181998No Hit
CGTAGTTGGATTTCGGGATGGCCCG128990.13936596319934724No Hit
TGAAAATCTTTACTCTGAAGTGGAA128820.1391822883893318No Hit
GAGACCGAGTGTGCTCTTAACT128200.138512415552805No Hit
AGTGTGCTCTTAACTG127720.13799380432452615No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACCG126810.1370106038709142No Hit
GACTGCTGCCGGTCGTTGGGGCGGT126070.13621107822731768No Hit
GAATCGGCCGTGGATTGGT125610.1357140758002171No Hit
GGAATCGGCCGTGGATTGGC124000.13397456730536522No Hit
GGAAGGAATCGGCCGTGGATTGGCT123670.13361802208592352No Hit
GGAAGGAATCGGCCGTGGATTGGC119510.12912339144084028No Hit
GGAAGGAATCGGCCGTGGATTGGCCCA119110.1286912154172746No Hit
GTTGGATCCGGCCCGGACTGGC114040.12321338431857942No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC112720.12178720344081263No Hit
ACTGCTGCCGGTCGTTGGGGC111830.12082561178837896No Hit
CGGACTGGCCGTGGTCGAGGA111310.12026378295774355No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGC110440.11932380010648819No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA109530.1183405996528762No Hit
GATCCGGCCCGGACTGGCC105890.1144077978384284No Hit
CGGTTAGGCGAACAAGAATAGAGGCC105100.11355425019188616No Hit
CCTTTCCGAGGTCGCGGTC100630.10872468312853951No Hit
CGTGGTGGACTGCTTGCAGTGC100360.10843296431263266No Hit
GGACTGGCCGTGGTCGAGGT99610.107622634268447No Hit
GGATCCGGCCCGGACTGGCC99170.10714724064252473No Hit
AGGAATCGGCCGTGGATT95560.10324685202984436No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA95450.10312800362336379No Hit
CAGCTGTGACCCTCGGGTA95020.10266341439803066No Hit
GGAAGGAATCGGCCGTGGATT93100.10058896948491533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)