FastQCFastQC Report
Wed 30 Apr 2025
1H4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10189645
Total Bases266.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA285693428.037620545171105No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC7096396.9643152435634414No Hit
GTTGGATCCGGCCCGGACTGGCC3764773.694701827198102No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC2207172.1660911641180824No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1486381.458716177060143No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1103711.0831682556163635No Hit
TTCTGCTGAACTACTTTGAGAACGAGT774990.7605662415128299No Hit
CTCGGGCTGAGACTTGAAGCG751880.7378863542351083No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT748350.7344220529763303No Hit
GTTGGATCCGGCCCGGACTGGCT658470.6462148583194017No Hit
TGAAAATCTTTGCTCTGAAGTGGA626140.6144865694535973No Hit
TGAAAATCTTTGCTCTGAAGTGGAT613450.6020327499142512No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT599370.5882148004174826No Hit
TTGTGTAACTCCCTAAGGAAGG580250.5694506530894845No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC461120.45253784601916947No Hit
CAGCTGTGACCCTCGGGTC412950.40526436396950044No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA406950.39937603321803655No Hit
ACCCGTAGATCCGAACTTGTGG371230.36432083747765504No Hit
ACCCGTAGATCCGAACTTGT362290.35554722465797384No Hit
TGAAAATCTTTGCTCTGAAGTGG322690.31668424169831233No Hit
TGATCATGGATTCTAAACTGGTAATGGCC307590.301865275973795No Hit
CACCCCTCATCCGCTACTTTGACCTCATT288310.2829441064924244No Hit
TAAAGAGGACTGATAATCTCAGGTTATC267010.26204053232472774No Hit
GTTGGATCCGGCCCGGACTGGCA264360.25943985290949784No Hit
TCAAGTCTAGGCTGGTTAGTTT255380.2506269845514736No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT232480.22815318885005317No Hit
TCGTCTGATTGGATACCCACGTACTTGATC216280.21225469582110074No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA186160.18269527544875214No Hit
AGGATGGACAGTGATTCAGAAA179060.17572741739285322No Hit
TCTCGGGCTGAGACTTGAAGCG173050.1698292727568036No Hit
TGAAAATCTTTGCTCTGAAGTGGAG171250.16806277353136445No Hit
TCTTGCTTTGGGCTAATGATAAGACGTGT166410.16331285339185025No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG165310.16223332608741522No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGC163120.1600840853631309No Hit
TCCGCTGTAGTATAGTGGTGAGTATTTCTGC156800.15388171030492231No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT156400.15348915492149137No Hit
CGGACTGGCCGTGGTCGAGGC156230.15332231888353323No Hit
TGAAAATCTTTGCTCTGAAGTGGGA152200.14936732339546666No Hit
TGAAAATCTTTGCTCTGAGGTGGAA150680.14787561293842916No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA149090.14631520528929123No Hit
CGAAAATCTTTGCTCTGAAGTGGAA139110.13652094847268967No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA138740.13615783474301607No Hit
TGAAAATCCTTGCTCTGAAGTGGAA138130.1355591877832839No Hit
TTCGTCTGGTGAATGATAGAGCTTCATC136120.13358659698154352No Hit
GTGGTCCGGACGGTCGAGGGTTATCAATCCT135980.1334492025973427No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCC130010.12759031349963615No Hit
GCACTGTGGTTCAGTGGTAGAATTCT117810.11561737430499297No Hit
CTCGGGCTGAGACTTGAAGCGGTT113490.11137777616393899No Hit
TTACCGTGACCGATCCCGT113120.11101466243426537No Hit
CTTTCCGAGGTCGCGGTC112640.11054359597414826No Hit
CTCGGGCTGAGACTTGAAGCA110520.10846305244196436No Hit
GTGGTCCGGACGGTCGAGGGTTATCAATCC109030.10700078363868418No Hit
CTCGGGCTGAGACTTGAAGC105410.1034481574186343No Hit
TGATCTTCTTGAATTTGCCCATTTACAATA105250.10329113526526194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)