FastQCFastQC Report
Wed 30 Apr 2025
1G9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7120598
Total Bases186.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA129745918.22120838727309No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA1651202.318906361516266No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1379031.9366772285136729No Hit
TCTCGGGCTGAGACTTGAAGCGGTT1238521.7393482963088212No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1151511.6171535031186988No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT632550.8883383109115274No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA568850.7988795323089437No Hit
CTCGGGCTGAGACTTGAAGCGGTT554620.778895255707456No Hit
ACCCGTAGATCCGAACTTGTGG543630.7634611587397575No Hit
CAGCTGTGACCCTCGGGTC524270.7362724310514369No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA484830.6808838246450649No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA452650.635690991121813No Hit
TCTCGGGCTGAGACTTGAAGCG404780.5684634914090081No Hit
TGAAAATCTTTGCTCTGAAGTGGAT333920.4689493775663224No Hit
GTTGGATCCGGCCCGGACT325830.4575879722461512No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC322310.45264456721191115No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT321820.451956422761122No Hit
TGAAAATCTTTGCTCTGAAGTGGA313890.44081971766977995No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC279120.3919895491923572No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT243800.3423869736783343No Hit
CTCGGGCTGAGACTTGAAGCG224780.3156757339762756No Hit
TTCTGCTGAACTACTTTGAGAACGAGT218570.3069545563448463No Hit
ACCCGTAGATCCGAACTTGT207160.2909306212764714No Hit
TGAAAATCTTTGCTCTGAAGTGG199210.2797658286565257No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA190050.26690174055606003No Hit
GTTGGATCCGGCCCGGACTGGCC177610.24943129776459788No Hit
TTGTGTAACTCCCTAAGGAAGG164850.2315114545154775No Hit
TCAAGTCTAGGCTGGTTAGTTT150160.21088116475610616No Hit
GCACTGTGGTTCAGTGGTAGAATTCT149460.20989810125497887No Hit
TCGTCTGATTGGATACCCACGTACTTGATC149060.20933635068290612No Hit
TAGGTGCTGCCCTGCAAATTCCCATATATC145420.20422442047704417No Hit
CGGACTGGCCGTGGTCGAGGC143110.20098031092332413No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA142100.19956189072884048No Hit
TCGAAGACTCTAAACGGCGGAAGCCTATT134570.18898693620957116No Hit
TCTCGGGCTGAGACTTGAAGCGGTC125590.17637563586653818No Hit
TCAGTCCCACCATCTCACCAAT109330.15354047511178134No Hit
CACCCCTCATCCGCTACTTTGACCTCATT108710.1526697617250686No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGT105960.14880772654206853No Hit
CTTTCCGAGGTCGCGGTC101170.14208076344149745No Hit
TGAAAATCTTTGCTCTGAAGTGGAG97370.13674413300680646No Hit
ACCCGTAGATCCGAACTTGTGA95440.13403368649655548No Hit
TCTCGGGCTGAGACTTGAAGCA95230.1337387674462173No Hit
CCTGACTGGCGCCGACACTCTGTCTCGTGCA94840.13319106063844638No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA94580.1328259227665991No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT92110.12935711298404992No Hit
GACCAAGATAGTCGAACTCGACCCTGTC88300.1240064387850571No Hit
TGAAAATCCTTGCTCTGAAGTGGAA85750.1204252788880934No Hit
TCTCGGGCTGAGACTTGAAGCGGT84850.11916134010092971No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC81550.11452689788132964No Hit
TACCAAGGTCTTGTAGTCTAGTACAACT79320.1113951384420241No Hit
AGCTGTGACCCTCGGGTC78930.1108474316342532No Hit
TGAAAATCTTTGCTCTGAAGTGGGA78740.11058060011251863No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT75750.10638151458627493No Hit
TGCTTCAAGGGCAGTTAGACCAAATCCTTC73720.10353063043300577No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC72470.10177515989527847No Hit
GCCGGTCTGCCGCGAGGTACGTTACT72170.1013538469662239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)