FastQCFastQC Report
Wed 30 Apr 2025
1G12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6074744
Total Bases146.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG3503195.766810914171856No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA2799164.607864956943041No Hit
CTCGGGCTGAGACTTGAAGCGGTT1829903.012308008370394No Hit
CGGACTGGCCGTGGTCGAGGC1506522.479972818607665No Hit
CAGCTGTGACCCTCGGGTC1386452.282318398931708No Hit
CGTGGTGGACTGCTTGCAGTGCT1145321.8853798612748125No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT1110591.8282087278081183No Hit
CAGCTGTGACCCTCGGGT825241.358476999195357No Hit
GTTGGATCCGGCCCGGACTGGCC758851.249188443167317No Hit
CTCGGGCTGAGACTTGAAGC585920.964518010964742No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC579890.9545916667434875No Hit
CGAGTGTGCTCTTAACTG569520.9375209885387762No Hit
CGGTTAGGCGAACAAGAATAGAGGCC447550.7367388650451773No Hit
TGAAAATCTTTGCTCTGAAGTGGAA394460.6493442357406337No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC390270.6424468257427803No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC318820.5248287006003874No Hit
TGAAAATCTTTGCTCTGAGGTGGAA279970.4608753883291214No Hit
GAGTGTGCTCTTAACTG276110.45452121109959526No Hit
CGGTTAGGCGAACAAGAATAGAGGC271130.4463233347775643No Hit
CTCGGGCTGAGACTTGAAGCGG270830.4458294868063576No Hit
CTCGGGCTGAGACTTGAAGCA267350.44010085034036006No Hit
GGACTGGCCGTGGTCGAGGC259990.427985113446756No Hit
GAGACCGAGTGTGCTCTTAACTG232190.38222186811493625No Hit
GAGACTTGAAGCGGTTGGA216500.3563936192208264No Hit
CTCGGGCTGAGACTTGAAGCGGTTG212920.35050036676442664No Hit
CTTTCCGAGGTCGCGGTC192920.3175771686839808No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA186470.306959437303037No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC184380.30351896310363036No Hit
GAGTGTGCTCTTAACTGA175950.28964183511272246No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT175180.2883742919866253No Hit
AGTGTGCTCTTAACTG174280.2868927480730052No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC171070.2816085747810937No Hit
GTTTACGGTTAGGCGAACAAGAATAGAGGCC164490.27077684261262697No Hit
GAGTGTGCTCTTAACT159760.26299050626660153No Hit
AGTGTGCTCTTAACT156980.25841418173341957No Hit
CGACTGCTGCCGGTCGTTGGGGC156280.25726186980060395No Hit
CTCGGGCTGAGACTTGAAGCGGT154990.2551383235244152No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA154860.2549243227368923No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA149340.24583752006668924No Hit
AGACTTGAAGCGGTTGGAT145450.23943395804004253No Hit
GACCAAGATAGTCGAACTCGACCCTGTC143520.2362568694252795No Hit
CGGACTGGCCGTGGTCGAGGT142870.235186865487665No Hit
GAGACTTGAAGCGGTTGGAT140720.23164762169401706No Hit
GGAAGGAATCGGCCGTGGATTGGCC121500.2000084283387086No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT119770.19716057170475004No Hit
TCTCGGGCTGAGACTTGAAGCG113920.18753053626621963No Hit
GCCGGGAAGTTGTTCAAACTTGATCA113790.18731653547869673No Hit
CTACCGATAGCCAACTCGAACGTTTC111250.18313528932248008No Hit
CCGATTGAATGGTTTAGTGAGGCCTC111210.1830694429263192No Hit
ACGGTTAGGCGAACAAGAATAGAGGC110200.18140682142325668No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT110150.18132451342805556No Hit
GTTGGATCCGGCCCGGACTGGC107340.17669880409775293No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG103050.1696367781094973No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC99780.16425383522334439No Hit
GAATCGGCCGTGGATTGGC98790.1626241369183623No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC94990.1563687292830776No Hit
GACTGGCCGTGGTCGAGGC94930.15626995968883625No Hit
CGTGGTGGACTGCTTGCAGTGCC92640.1525002535086252No Hit
GATCCGGCCCGGACTGGCC92480.15223686792398167No Hit
GAGACCGAGTGTGCTCTTAACT92380.15207225193357943No Hit
CTCGGGCTGAGACTTGAAGCGGTC87670.14431883878563442No Hit
ACCGATTGAATGGTTTAGTGAGGCCTC87000.1432159116499395No Hit
CTACCGATTGAATGGTTTAGTGAGGCCTC86910.14306775725857748No Hit
GACCGTGGTGGACTGCTTGCAGTGCT86690.14270560207969257No Hit
GGAAGGAATCGGCCGTGGATTGGCCCA86260.14199775332096298No Hit
CTACCGATAGCCAACTCGAACGTTTCCG84030.13832681673499328No Hit
CTGACTGGCGCCGACACT79930.13157756112850189No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC79840.13142940673713988No Hit
TTGGATCCGGCCCGGACTGGCC79220.13040878759664604No Hit
TCTCGGGCTGAGACTTGAAGCGGTT78840.12978324683311757No Hit
GAGACCGAGTGTGCTCTTAACTGAGTGTG78750.12963509244175556No Hit
GGAAGGAATCGGCCGTGGATT75500.12428507275368311No Hit
GACCAAGATAGTCGAACTCGACCCTGTT75500.12428507275368311No Hit
AGACTTGAAGCGGTTGGATCCGGCCC75330.12400522556999932No Hit
TTACGGTTAGGCGAACAAGAATAGAGGCC73300.12066352096483407No Hit
AGACTTGAAGCGGTTGGA72810.11985690261186316No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT72810.11985690261186316No Hit
CTTTCCGAGGTCGCGGTCGGA70380.11585673404508898No Hit
CGTGGTGGACTGCTTGCAGT69040.11365087977369911No Hit
GTTGGATCCGGCCCGGACTGGCT68760.11318995500057287No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA68070.1120541046667975No Hit
GACTTGAAGCGGTTGGAT67370.11090179273398187No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC66590.10961778800884449No Hit
CGTAGTTGGATTTCGGGATGGCCCG65990.10863009206643112No Hit
TGGGGGTAGTCGTTCTATGT65310.10751070333169595No Hit
GAGACCGAGTGTGCTCTTAACTGA64180.10565054264015075No Hit
GGAGGCCGGGAAGTTGTTCAAACTTGATCA63800.1050250018766223No Hit
GGATCCGGCCCGGACTGGCC63660.10479453949005917No Hit
AACAGTAGTGGGATAGCA63060.10380684354764579No Hit
GAGACCGAGTGTGCTCTTAACTGAGTGT63010.10372453555244467No Hit
CGACTGCTGCCGGTCGTTGGGGCG62410.1027368396100313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)