FastQCFastQC Report
Wed 30 Apr 2025
1F9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7114109
Total Bases181.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA139257919.574889842143268No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3800175.341737102987879No Hit
CTCGGGCTGAGACTTGAAGCG1415921.9902984337181227No Hit
CGGACTGGCCGTGGTCGAGGC954941.3423184828908301No Hit
TTCTGCTGAACTACTTTGAGAACGAGT721711.0144770061858766No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC662710.9315432192562695No Hit
CAGCTGTGACCCTCGGGTC642400.9029943173488064No Hit
TTGTGTAACTCCCTAAGGAAGG499390.7019712517758724No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC487940.6858764744819063No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT472390.6640185018250353No Hit
CTCGGGCTGAGACTTGAAGCGGTT381680.5365113185642784No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA357760.5028879934226478No Hit
ACCCGTAGATCCGAACTTGTGG331670.4662143917108945No Hit
TGAAAATCTTTGCTCTGAAGTGGA293220.41216686446609124No Hit
GTTGGATCCGGCCCGGACTGGCC274120.38531880801938795No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGT267320.3757603376613993No Hit
CGACTGCTGCCGGTCGTTGGGGCG240800.33848230326524376No Hit
CTCGGGCTGAGACTTGAAGC222340.31253386755811585No Hit
ACCCGTAGATCCGAACTTGT213620.30027653498140106No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT195060.2741875335337145No Hit
TGAAAATCTTTGCTCTGAAGTGGAT193220.27160112390743524No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT185030.2600887897556813No Hit
TGAAAATCTTTGCTCTGAAGTGG183980.2586128494798154No Hit
TCAAGTCTAGGCTGGTTAGTTT180470.2536789919862066No Hit
CAGCTGTGACCCTCGGGT164450.23116036034870985No Hit
TGAAAATCTTTGCTCTGAAGTGGAG162690.22868640331487755No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA158670.22303566054441953No Hit
CGACTGCTGCCGGTCGTTGGGGC155910.21915604610500064No Hit
CTCGGGCTGAGACTTGAAGCA154470.217131899440956No Hit
GGACTGGCCGTGGTCGAGGC130240.18307282050359364No Hit
CGGACTGGCCGTGGTCGAGGT129590.18215914318996237No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT126510.17782971838075576No Hit
TTACCGTGACCGATCCCGT124040.17435774458895698No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA119770.16835558746710236No Hit
CGAGTGTGCTCTTAACTG118880.1671045523761303No Hit
CACCCCTCATCCGCTACTTTGACCTCATT117920.1657551212667672No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA116660.16398399293572813No Hit
TAAAGAGGACTGATAATCTCAGGTTATC116350.1635482391399963No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT114310.1606806980325997No Hit
CTTTCCGAGGTCGCGGTC112450.15806617525820874No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG110180.15487533294752723No Hit
TGATCTGCGCATAGGAATTGCCCTGGACTGC98750.13880866880167284No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC94480.13280651167981822No Hit
GTTCTATTTTGTTGGTTTCTGGAACC92580.1301357626092038No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC89130.12528624455993015No Hit
CTCGGGCTGAGACTTGAAGCGG86220.12119578150967324No Hit
TCTATTTTGTTGGTTTCTGGAACC82630.11614947142361749No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC79310.11148268883707012No Hit
AGGATGGACAGTGATTCAGAAA76550.10760307439765121No Hit
CCCCCCTCGTCCACTCACATGACCACTT76150.10704081143541658No Hit
GGAAGGAATCGGCCGTGGATTGGCC74120.10418732690207586No Hit
TGAAAATCTTTGCTCTGAAGTGGGA72910.10248648144131611No Hit
GTGGTCCGGACGGTCGAGGGTTATCAATCCT72800.1023318591267016No Hit
TAGGAATTGCCCTGGACTGCAGTAGAGGC72330.10167120014607592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)