FastQCFastQC Report
Wed 30 Apr 2025
1F2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9560183
Total Bases245.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA276496828.92170578743106No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA1845891.9308103202626978No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1418391.4836431478351408No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1225221.2815863462027872No Hit
TCTCGGGCTGAGACTTGAAGCGGTT1169861.2236795048797706No Hit
TCTCGGGCTGAGACTTGAAGCG667750.6984698933064357No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA534460.559047875966391No Hit
TGAAAATCTTTGCTCTGAAGTGGA532580.5570813864127915No Hit
CAGCTGTGACCCTCGGGTC502410.5255234130978454No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT486040.5084003099103857No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA483830.5060886386798245No Hit
CTCGGGCTGAGACTTGAAGCGGTT449770.4704617055970581No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA362500.37917684211693437No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA349000.3650557735139589No Hit
CTCGGGCTGAGACTTGAAGCG288440.3017097057660925No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT287550.3007787612433779No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA266020.2782582718343362No Hit
TGAAAATCTTTGCTCTGAAGTGGAT260020.2719822413441249No Hit
TTCTGCTGAACTACTTTGAGAACGAGT259630.27157429936226113No Hit
TGAAAATCTTTGCTCTGAAGTGG215170.22506891342979524No Hit
ACCCGTAGATCCGAACTTGTGG203820.2131967557524788No Hit
GTTGGATCCGGCCCGGACTGGCC186130.1946929258571724No Hit
CACCCCTCATCCGCTACTTTGACCTCATT184110.1925799955921346No Hit
TTACCGTGACCGATCCCGT173340.18131452086220526No Hit
TGAAAATCCTTGCTCTGAAGTGGAA168550.1763041565208532No Hit
GTTGGATCCGGCCCGGACT168130.1758648343865384No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC167080.17476652905075143No Hit
ACCGATTGAATGGTTTAGTGAGGCCT163950.17149253314502452No Hit
GACCAAGATAGTCGAACTCGACCCTGTC160730.16812439678194444No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAAA152990.16002831744957183No Hit
TGAAAACCTTTGCTCTGAAGTGGAA142550.14910802439660412No Hit
TGAAAATCTTTGCTCTGAAGTGGGA141900.1484281210934979No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT139600.14602230940558356No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAA134520.14070860359053797No Hit
CCTGACTGGCGCCGACACTCTGTCTCGTGCA128420.13432797259215645No Hit
GAGACCGAGTGTGCTCTTAACTGAGT128000.13388865045784165No Hit
TGAAAATCTTTGCTCTGAAGTGGAG127800.13367944944150126No Hit
TCCGCTGTAGTATAGTGGTGAGTATTTCTGC118410.12385746172432055No Hit
TGAAAATCTTTGCTCTGAGGTGGAA118050.12348089989490786No Hit
AACTTTTGACGGTGGATCTCTAGGCTCACG110440.1155208012231565No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA110120.11518607959701191No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC109110.11412961446449299No Hit
AAAGACGAACTACTGCGAAAGCATTTGC103910.10869038803964318No Hit
TTGTGTAACTCCCTAAGGAAGG102890.10762346285630724No Hit
TGAAAATCTTTGCTCTGGAGTGGAA102550.1072678211285286No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA102150.10684941909584786No Hit
GCCGGTCTGCCGCGAGGTACGTTACT97800.10229929699044464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)