FastQCFastQC Report
Wed 30 Apr 2025
1E8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7397599
Total Bases181.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA173704923.481253850066757No Hit
GTTGGATCCGGCCCGGACTGGCC2633903.5604795555963498No Hit
CTCGGGCTGAGACTTGAAGCG2326303.144668966241614No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1644152.222545450219727No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1231851.6652024528499045No Hit
TCTCGGGCTGAGACTTGAAGCG1015991.3734050737272998No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT844231.141221631505033No Hit
CTCGGGCTGAGACTTGAAGC642620.8686872592039661No Hit
CGGACTGGCCGTGGTCGAGGC639890.8649968726339451No Hit
CAGCTGTGACCCTCGGGTC637890.8622932927291679No Hit
CTCGGGCTGAGACTTGAAGCGGTT618400.835946906557114No Hit
CGGACTGGCCGTGGTCGAGGT569150.7693712514019752No Hit
CGAGTGTGCTCTTAACTG499220.67484058003144No Hit
CGGACTGGCCGTGGTCGAGG496710.6714475872509446No Hit
GTTGGATCCGGCCCGGACTGGCT493510.667121859403301No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA434850.5878258607961854No Hit
TCTCGGGCTGAGACTTGAAGCGGTT387520.5238456423496326No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA360310.48706343774513866No Hit
TGAAAATCTTTGCTCTGAAGTGGA358750.48495464541941247No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA354480.4791825023227131No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT317280.4288959160938569No Hit
GGATCCGGCCCGGACTGGCC296340.40058943449083956No Hit
CTCGGGCTGAGACTTGAAGCA296240.40045425549560065No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC290050.39208667569031513No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT289410.39122153012078653No Hit
CAGCTGTGACCCTCGGGT276940.37436470941450056No Hit
CTTTCCGAGGTCGCGGTC273170.36926846129399554No Hit
GTTGGATCCGGCCCGGACTGGC266470.36021146861299186No Hit
TCTCGGGCTGAGACTTGAAGC250580.3387315262695369No Hit
TTCTGCTGAACTACTTTGAGAACGAGT244750.3308505908471113No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA225660.30504492065601285No Hit
TTCCGAGGTCGCGGTCGGACCG217190.29359525975928136No Hit
GATCCGGCCCGGACTGGCC203310.27483241522012747No Hit
CTCGGGCTGAGACTTGAAGCGGT202670.2739672696505988No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC196760.2659781910319821No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC196570.2657213509410283No Hit
TGAAAATCTTTGCTCTGAAGTGGAT194880.26343682592149154No Hit
TTGGATCCGGCCCGGACTGGCC186790.25250084520666777No Hit
GAGTGTGCTCTTAACTGA186560.25218993351761837No Hit
ACCCGTAGATCCGAACTTGTGG182260.24637723672234735No Hit
TGGATCCGGCCCGGACTGGCC172210.23279174770084185No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA170650.23068295537511563No Hit
TGAAAATCTTTGCTCTGAAGTGG167280.226127423235566No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGAT164080.2218016953879225No Hit
GAGTGTGCTCTTAACTG163420.220909514019346No Hit
TCTCGGGCTGAGACTTGAAGCGGT159920.21617824918598588No Hit
GTTGGATCCGGCCCGGACT154810.2092706025292801No Hit
GTTGGATCCGGCCCGGACTGGCA153850.20797288417498705No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC148760.20109227331732904No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT148600.20087598692494685No Hit
GAGACTTGAAGCGGTTGGAT147980.20003787715446594No Hit
TTGTGTAACTCCCTAAGGAAGG136550.18458691799866417No Hit
CGGACTGGCCGTGGTCGAGGTG133820.18089653142864326No Hit
TCTCGGGCTGAGACTTGAAGCA125820.17008221180953442No Hit
TTCCGAGGTCGCGGTCGGACCGGGA121700.16451283720569337No Hit
TCCGCTGTAGTATAGTGGTGAGTATTTCTGC117400.15870014041042235No Hit
TTCCGAGGTCGCGGTCGGACC116560.1575646368504159No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG114720.1550773433380209No Hit
CTCGGGCTGAGACTTGAAGCGG113540.15348223119420235No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA113470.15338760589753514No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG112690.15233320973467201No Hit
GAATCGGCCGTGGATTGGC108860.14715585421702365No Hit
GAGACTTGAAGCGGTTGGA104600.1413972290198482No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA103870.14041042235460452No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT103620.14007247486650737No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA99880.135016780444574No Hit
CGACTGCTGCCGGTCGTTGGGGCG98490.13313779241075382No Hit
TGAAAATCTTTGCTCTGAAGTGGAG98170.13270521962598947No Hit
AGACTTGAAGCGGTTGGAT97320.13155619816645914No Hit
CTTTCCGAGGTCGCGGTCGGA96730.13075864209454988No Hit
GCACTGTGGTTCAGTGGTAGAATTCT95800.12950147743882845No Hit
CGGACTGGCCGTGGTCGAGGTGA95120.12858226027120423No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGGA93820.12682493333309902No Hit
TGAAAATCTTTGCTCTGAAGTGGGA93490.1263788426488108No Hit
GAGACCGAGTGTGCTCTTAACTG93380.12623014575404803No Hit
TGAAAATCCTTGCTCTGAAGTGGAA90070.12175572101164175No Hit
ACCCGTAGATCCGAACTTGT87430.11818699553733582No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC87190.11786256594876257No Hit
TAAAGAGGACTGATAATCTCAGGTTATC86910.11748406476209375No Hit
GAGTGTGCTCTTAACT86330.11670002658970838No Hit
CACCCCTCATCCGCTACTTTGACCTCATT86280.11663243709208893No Hit
TGAAAATCTTTGCTCTGAGGTGGAA85510.1155915588287497No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA85500.11557804092922581No Hit
CGACCAAGATAGTCGAACTCGACCCTGT85280.11528064713970032No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA85270.11526712924017643No Hit
GGAAGGAATCGGCCGTGGATTGGCC83650.11307722951730691No Hit
TATTAATGCTGTAGGTGAAAGACA83040.11225263764634984No Hit
TGGGGGTAGTCGTTCTATGT80210.1084270720810901No Hit
TATTAATGCTGTAGGTGAAAGACC79440.10738619381775087No Hit
GGATCCGGCCCGGACTGGC78330.10588570697059951No Hit
GGTTGGATCCGGCCCGGACTGGC77650.10496648980297527No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC76260.1030875017691551No Hit
CTCGGGCTGAGACTTGAAGT75430.10196551610867255No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGG74510.10072186935247504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)