FastQCFastQC Report
Wed 30 Apr 2025
1E6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12643536
Total Bases325.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA518231040.987821761254125No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4914423.886903157471138No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC2405911.9028774861715898No Hit
GTTGGATCCGGCCCGGACTGGCC1845851.4599159602187237No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT939630.7431702650271254No Hit
TGAAAATCTTTGCTCTGAAGTGGA922330.7294873839090584No Hit
TTCTGCTGAACTACTTTGAGAACGAGT921930.729171016715577No Hit
CAGCTGTGACCCTCGGGTC754450.5967080728049495No Hit
CTCGGGCTGAGACTTGAAGCG681690.539160880310698No Hit
ACCCGTAGATCCGAACTTGTGG586280.46369939548556666No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC568520.4496526920949962No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA544940.4310028460392725No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA524990.4152240322643918No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC427750.33831516752908364No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT419590.3318612767820648No Hit
AGGATGGACAGTGATTCAGAAA418530.3310229037193393No Hit
TATTAATGCTGTAGGTGAAAGACA402690.31849476285747913No Hit
TGAAAATCTTTGCTCTGAAGTGG394990.31240469438296375No Hit
CACCCCTCATCCGCTACTTTGACCTCATT377140.29828680837386No Hit
TGAAAATCCTTGCTCTGAAGTGGAA296940.23485518608085584No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA279440.22101412136604823No Hit
TGAAAATCTTTGCTCTGAAGTGGGA258440.20440484370827908No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC231730.18327942436356412No Hit
TATTAATGCTGTAGGTGAAAGACC220790.17462678162185008No Hit
CGGACTGGCCGTGGTCGAGGC220070.17405732067358373No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAA215820.17069591924284472No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA212240.16786443286118694No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA209240.16549167891007707No Hit
TGAAAATCTTTGCTCTGAGGTGGAA208500.16490639960213663No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA206440.16327710855570784No Hit
TGAAAATCTTTGCTCTGAAGTGGAG198420.15693394632640742No Hit
ACCCGTAGATCCGAACTTGT193320.1529002646095206No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT189450.14983941201258888No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA187250.14809939244844164No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAA181860.14383634451628088No Hit
TAAGTTGAAGATTGTAGCAGAAGCAAAAGC173260.13703444985643257No Hit
TGAAAATCTTTGCCCTGAAGTGGAA172930.13677344692181048No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT171470.1356187066656037No Hit
TGAAAATCTTTGCTCTGGAGTGGAA169200.1338233228425972No Hit
TTGTGTAACTCCCTAAGGAAGG164660.13023255519658425No Hit
CGAAAATCTTTGCTCTGAAGTGGAA155540.1230193831852102No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAAA154460.12216519176281065No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAA152890.12092345052839648No Hit
AAACTGACGAGCTTTTCGATTTCAAAATG146910.1161937609858508No Hit
TATTAATGCTGTAGGTGAAAGACCA146260.11567966429644365No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG143440.11344927558240037No Hit
TGAAAATCTTTGCTCCGAAGTGGAA142850.11298263397201541No Hit
TGAAAATCTTTGCTCTGAAGTGGAAA141720.11208889665043069No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC136890.1082687627891438No Hit
TCTCGGTGGGACCTCCATT136130.1076676651215293No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA131850.10428253615127919No Hit
CTTGATGAAGAAATGCTATGTAAGCTTCACC131830.10426671779160514No Hit
GACCAAGATAGTCGAACTCGACCCTGTC129770.10263742674517634No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAAA127820.10109513667695494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)