FastQCFastQC Report
Wed 30 Apr 2025
1D11-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D11-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7583574
Total Bases181.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCGGTTGGA3985955.256030995411925No Hit
CTCGGGCTGAGACTTGAAGC2952643.8934676446751886No Hit
CTCGGGCTGAGACTTGAAGCG2897923.8213116928772632No Hit
CAGCTGTGACCCTCGGGTC2888003.8082307893349494No Hit
CGTGGTGGACTGCTTGCAGTGCT2204942.9075209129626742No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT2167512.858164237600899No Hit
CAGCTGTGACCCTCGGGT2041002.6913431582522964No Hit
CGGACTGGCCGTGGTCGAGGC1767812.331104041445366No Hit
CTCGGGCTGAGACTTGAAGCGGTT941331.2412748922869348No Hit
TGAAAATCTTTGCTCTGAAGTGGAA834941.1009848390745578No Hit
CGATCAAGATAGTCGAACTCGACCCTGTC799111.0537379868647685No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC772981.019281937513895No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC729210.9615650879123747No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC728680.9608662089932795No Hit
CGACTGCTGCCGGTCGTTGGGGCG706230.9312627528919741No Hit
GGAAGGAATCGGCCGTGGATTGGCC511610.6746291392422623No Hit
GACTTGAAGCGGTTGGAT379300.5001599509677099No Hit
CTCGGGCTGAGACTTGAAGCA373680.4927491971463587No Hit
GAGACTTGAAGCGGTTGGAT368810.486327422927501No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC362740.4783232813446536No Hit
GGACTGGCCGTGGTCGAGGC360370.47519810580077415No Hit
GAGACTTGAAGCGGTTGGA355920.4693301601593127No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTC355050.4681829438204203No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT305200.40244876624135273No Hit
CTTTCCGAGGTCGCGGTC273660.3608588773578263No Hit
CTCGGGCTGAGACTTGAAGT250280.3300290865494291No Hit
CTCGGGCTGAGACTTGAAGCGGTTG247000.32570394908785755No Hit
CGGACTGGCCGTGGTCGAGGT238910.3150361557756277No Hit
GGAAGGAATCGGCCGTGGATT235380.3103813584465583No Hit
CGTGGTGGACTGCTTGCAGTGCC232900.3071111325609798No Hit
CGAGTGTGCTCTTAACTG213610.2816745771848471No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC213290.28125261255444994No Hit
CGACTGCTGCCGGTCGTTGGGGC212820.28063285200355403No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC206530.27233860973730856No Hit
TGGGGGTAGTCGTTCTATGTAGATCGGGT200170.26395206270816374No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTT196680.259350010957894No Hit
CGATCAAGATAGTCGAACTCGACCCTGTT196070.2585456408811993No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA194510.2564885633080128No Hit
CAGCTGTGACCCTCGGGC189670.2501063482732548No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC185490.24459443528869104No Hit
CTCGGGCTGAGACTTGAAGCGGT185000.2439483019483953No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA181550.23939899577692525No Hit
CTCGGGCTGAGACTTGAAGCGG175640.2316058365092765No Hit
TGGGGGTAGTCGTTCTATGT167780.22124133027514467No Hit
CGACCAAGATAGTCGAACTCGACCCTGT167480.2208457384341473No Hit
GTTGGATCCGGCCCGGACTGGCC158150.2085428321791282No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATC156040.2057605028974465No Hit
CTTTCCGAGGTCGCGGTCGGA150610.1986002905753936No Hit
AGACTTGAAGCGGTTGGAT149860.19761131097290013No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT148010.19517182795341614No Hit
GAAGGAATCGGCCGTGGATT138330.18240739788390012No Hit
AACAGTAGTGGGATAGCA133910.17657901142653845No Hit
GGAAGGAATCGGCCGTGGAT132930.17528674474594697No Hit
TGCGTTCGCGCTCGTCCCCTT131960.1740076644600554No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAC131890.173915359697156No Hit
CCTGACTGGCGCCGACACT131260.17308461683106144No Hit
GGAAGGAATCGGCCGTGGATTGGCCCA130580.17218794199146736No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT130430.17199014607096866No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTCA130310.17183190933456968No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG125170.16505410245881427No Hit
GAATCGGCCGTGGATTGGC122360.16134872554813864No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGT118950.15685216495546822No Hit
CGATCAAGATAGTCGAACTCGACCCTGT117330.15471596901408227No Hit
CCGATCAAGATAGTCGAACTCGACCCTGT114110.1504699499207102No Hit
CGGACTGGCCGTGGTCGAGGCGAGG113600.14979744379101462No Hit
CAGCTGTGACCCTCGGGTT112710.14862385466272235No Hit
CGACTGCTGCCGGTCGTTGGGGCA110960.14631623559023754No Hit
CGTGGTGGACTGCTTGCAGT108320.14283502738946044No Hit
CGATCAAGATAGTCGAACTCGACCCTGTCA106950.1410284913155723No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGATCCG106820.14085706818447344No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCT104730.13810111169219155No Hit
GAGTGTGCTCTTAACTG102810.13556932390980822No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT101040.13323533204792357No Hit
CCGGGAAGGAATCGGCCGTGGATT99650.13140242318463563No Hit
TCTCGGGCTGAGACTTGAAGCG93320.12305543533959054No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT91910.12119615368690276No Hit
TCTCGGGCTGAGACTTGAAGC86540.11411505973304935No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT84590.11154371276656627No Hit
CGTGGTGGACTGCTTGCAGTGCA84480.11139866242486722No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGAT83460.11005365016547608No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA79150.10437031404981346No Hit
CTTTCCGAGGTCGCG78970.10413295894521502No Hit
GAGACCGAGTGTGCTCTTAACTG76960.10148249361053244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)