FastQCFastQC Report
Wed 30 Apr 2025
1C8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664436
Total Bases169.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA254143238.13423971660917No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1820682.7319341051515837No Hit
GTTGGATCCGGCCCGGACTGGCC1760392.641468835472349No Hit
CTCGGGCTGAGACTTGAAGCG1085471.628749979743222No Hit
CAGCTGTGACCCTCGGGTC831821.248147630197064No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC817031.225955204611463No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA709161.0640960465371714No Hit
TGAAAATCTTTGCTCTGAAGTGGA515980.7742290570424865No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA391900.5880467604460452No Hit
CGGACTGGCCGTGGTCGAGGC371640.5576465885485283No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT343940.5160826812651513No Hit
CTCGGGCTGAGACTTGAAGCGGTT329270.49407031592770945No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT281610.4225563873672131No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA269750.4047604328408286No Hit
CGGACTGGCCGTGGTCGAGG259540.3894403067266307No Hit
TTCTGCTGAACTACTTTGAGAACGAGT256750.3852539059569332No Hit
CGGACTGGCCGTGGTCGAGGT243680.3656423439282784No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC233990.3511024788894364No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA233760.35075736341379826No Hit
TGAAAATCTTTGCTCTGAAGTGG221940.3330214289701334No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC206060.30919345613042126No Hit
CTCGGGCTGAGACTTGAAGC184620.2770226917926738No Hit
CACCCCTCATCCGCTACTTTGACCTCATT182670.2740967127600895No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT170930.25648081848186405No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC170600.2559856527994267No Hit
TCCGCTGTAGTATAGTGGTGAGTATTTCTGC160950.2415058078433044No Hit
GTTGGATCCGGCCCGGACTGGC153250.22995194191976634No Hit
ACCCGTAGATCCGAACTTGTGG151560.22741609342485994No Hit
TCTCGGGCTGAGACTTGAAGCG135530.203363045274949No Hit
TGAAAATCCTTGCTCTGAAGTGGAA129950.19499024373555393No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA128990.19354976175028166No Hit
TGAAAATCTTTGCTCTGAAGTGGGA126800.19026366222137928No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAA126750.19018863711797968No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAAA122000.1830612522950179No Hit
TATTAATGCTGTAGGTGAAAGACA121560.18240103138510144No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA120190.18034534355195248No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA119470.17926498206299826No Hit
TGAAAATCTTTGCTCTGAGGTGGAA117700.17660909340265252No Hit
GTTGGATCCGGCCCGGACTGGCT116040.1741182599697859No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG111860.16784616132557953No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT108680.16307456474936513No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT103910.15591716988504353No Hit
TGAAAATCTTTGCTCTGAAGTGGAG103260.15494184354084875No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAA103000.15455171300317086No Hit
TTCCGAGGTCGCGGTCGGACCG102550.15387648707257448No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA97940.1469591725391316No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC96330.14454336420966454No Hit
TATTAATGCTGTAGGTGAAAGACC93530.1403419584192871No Hit
TGAAAATCTTTGCTCTGAAGTGGAT92020.13807620029661924No Hit
TTGGATCCGGCCCGGACTGGCC90560.13588546727735099No Hit
CACCCCTCGCCTCTACTTGACCTCATT90310.135510341760353No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAA88840.13330460372040484No Hit
TGAAAATCTTTGCTCTGGAGTGGAA84160.12628225404220253No Hit
CAGCTGTGACCCTCGGGT80410.12065537128723271No Hit
TGAAAATCTTTGCCCTGAAGTGGAA80040.12010018552207567No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA79420.11916987423992068No Hit
CCCCCCTCGTCCACTCACATGACCACTT77290.11597380483509781No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTCA77250.11591378475237814No Hit
CTCGGGCTGAGACTTGAAGCGG76560.1148784383254637No Hit
CGAAAATCTTTGCTCTGAAGTGGAA75730.1136330216090304No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT74550.11186242916879988No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAA74140.11124722332092318No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG73130.10973171623225131No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAA72880.10935659071525333No Hit
CGAGTGTGCTCTTAACTG72810.10925155557049389No Hit
TCTCGGGCTGAGACTTGAAGCGGTT71570.10739093300618385No Hit
TGGAAATCTTTGCTCTGAAGTGGAA68970.10348962762940479No Hit
TGAAGATCTTTGCTCTGAAGTGGAA68510.10279939667812851No Hit
CGGACTGGCCGTGGTCGAGGTGA68410.10264934647132931No Hit
GGATCCGGCCCGGACTGGCC68160.10227422095433131No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC67880.10185408037529359No Hit
TTACCGTGACCGATCCCGT67770.10168902514781446No Hit
CTCGGGCTGAGACTTGAAGCGGT67250.10090876407245865No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAAA67130.10072870382429962No Hit
TGAAAATCTTTGCTCCGAAGTGGAA67030.10057865361750042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)