FastQCFastQC Report
Wed 30 Apr 2025
1C7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7009278
Total Bases177.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA188043326.82777027819413No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1784282.545597421018256No Hit
CTCGGGCTGAGACTTGAAGCG1332681.9013085227893658No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1283711.831443980392845No Hit
CGGACTGGCCGTGGTCGAGGC1203781.7174094107838211No Hit
CAGCTGTGACCCTCGGGTC786791.1224979234665824No Hit
CGTGGTGGACTGCTTGCAGTGCT593240.8463639193651614No Hit
TGAAAATCTTTGCTCTGAAGTGGA382290.5454056751636902No Hit
CTCGGGCTGAGACTTGAAGCGGTT368570.525831619176754No Hit
TTCTGCTGAACTACTTTGAGAACGAGT355100.5066142333061979No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA340930.4863981711097776No Hit
GTTGGATCCGGCCCGGACTGGCC329350.4698772113190546No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT323880.4620732691726594No Hit
ACCCGTAGATCCGAACTTGTGG297540.4244945057108592No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA268390.3829067701409475No Hit
TGAAAATCTTTGCTCTGAAGTGGAT248400.3543874276352001No Hit
CTCGGGCTGAGACTTGAAGC240360.34291691669241825No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC211390.3015859836063001No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT204560.2918417560267976No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA200730.2863775698438555No Hit
CAGCTGTGACCCTCGGGT198550.28326740642902165No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT198340.28296780353126244No Hit
TGAAAATCTTTGCTCTGAAGTGG197640.2819691272053983No Hit
GTTGGATCCGGCCCGGACTGGC182980.2610539915808732No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA179120.25554700498396554No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC174390.24879880638205534No Hit
CGGACTGGCCGTGGTCGAGGT161280.23009502547908642No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGT156890.22383189823545308No Hit
GGACTGGCCGTGGTCGAGGC150590.21484381130267624No Hit
CTCGGGCTGAGACTTGAAGCA146260.20866628488697408No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG142950.20394397254610247No Hit
TTACCGTGACCGATCCCGT140610.20060554025678537No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC137210.19575482667401695No Hit
ACCCGTAGATCCGAACTTGT136520.19477041715280805No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA135950.19395720928746157No Hit
CTCGGGCTGAGACTTGAAGCGG132930.1896486342815908No Hit
CGGTTAGGCGAACAAGAATAGAGGC130040.1855255277362376No Hit
GAGACCGAGTGTGCTCTTAACTG128020.18264363319588695No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGC105670.15075732479151205No Hit
CGGTTAGGCGAACAAGAATAGAGGCC105310.15024371982392482No Hit
TTGTGTAACTCCCTAAGGAAGG102060.1456070083109844No Hit
TGAAAATCTTTGCTCTGAAGTGGGA100940.14400912618960185No Hit
TGAAAATCCTTGCTCTGAAGTGGAA96470.13763186450872686No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC95740.13659038776889718No Hit
GGAAGGAATCGGCCGTGGATTGGCC93270.13306648701906246No Hit
TGAAAATCTATGCTCTGAAGTGGAA92240.13159700613957673No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC91440.1304556617671606No Hit
TAAAGAGGACTGATAATCTCAGGTTATC91280.1302273928926774No Hit
TGAAAATCTTTGCTCTGAAGTGGAG89080.1270886958685331No Hit
GTGTGTGATGACTGCATAGTTCAACTGAACA87960.12549081374715057No Hit
TATTAATGCTGTAGGTGAAAGACA86330.12316532458835276No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA84610.12071143418765813No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAAA83880.11966995744782845No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT81970.116944997758685No Hit
CGAGTGTGCTCTTAACTG81740.11661686125161537No Hit
CTTTCCGAGGTCGCGGTC80090.11426283848350716No Hit
TGATCTGCGCATAGGAATTGCCCTGGACTGC79710.1137206999066095No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGCGAG78010.11129534311522529No Hit
TATTAATGCTGTAGGTGAAAGACC75900.10828504733297782No Hit
AGACTTGAAGCGGTTGGAT75330.10747183946763132No Hit
TGAAAATCTTTGCTCTGAGGTGGAA74420.10617356024400801No Hit
GACTGGCCGTGGTCGAGGC72880.10397647232710701No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA72380.10326313209434695No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAA71730.10233578979175888No Hit
TCAAGTCTAGGCTGGTTAGTTT70840.10106604417744595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)