FastQCFastQC Report
Wed 30 Apr 2025
1C3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12142915
Total Bases308.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA449889137.04951405819772No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC2723232.2426493144356194No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2163961.7820762148133293No Hit
CAGCTGTGACCCTCGGGTC1456721.1996460487452971No Hit
CTCGGGCTGAGACTTGAAGCGGTT1100490.9062815641878411No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC1061610.8742628932179795No Hit
GTTGGATCCGGCCCGGACTGGCC823570.6782308860763664No Hit
TGAAAATCTTTGCTCTGAAGTGGA787920.6488722024324473No Hit
CTCGGGCTGAGACTTGAAGCG784480.6460392747540438No Hit
CGGACTGGCCGTGGTCGAGG749540.6172652942065393No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA734250.6046735894964265No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT716780.5902865992226742No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA691750.5696737562603378No Hit
CGGACTGGCCGTGGTCGAGGT587670.4839612234788764No Hit
TTCTGCTGAACTACTTTGAGAACGAGT576940.4751247949936238No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAG518350.4268744366570959No Hit
GGATCCGGCCCGGACTGGCC518040.4266191437558444No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT470400.3873863895119088No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA456530.3759640909946253No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA449860.3704711759902791No Hit
CGGACTGGCCGTGGTCGAGGC440420.3626970953844279No Hit
TTCCGAGGTCGCGGTCGGACCG433040.35661947728366705No Hit
CGTGGTGGACTGCTTGCAGTGCT422260.34774187252401917No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA417280.3436407155942375No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC409920.3375795680032348No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT391920.3227561092208914No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC351890.28979038393993534No Hit
ACCCGTAGATCCGAACTTGTGG350260.28844803739464536No Hit
TGAAAATCTTTGCTCTGAAGTGG332830.2740939881404095No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGGA330550.2722163500279793No Hit
TGAAAATCCTTGCTCTGAAGTGGAA307170.2529623241206909No Hit
CGACCAAGATAGTCGAACTCGACCCTGT298010.24541883065145395No Hit
TGAAAATCTTTGCTCTGAAGTGGAT290830.23950591764827475No Hit
CTCGGGCTGAGACTTGAAGC285110.23479535185744116No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA270920.22310952518402707No Hit
CAGCTGTGACCCTCGGGT269660.222071883069263No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG261570.21540956187208757No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT258200.2126342809778377No Hit
GTTGGATCCGGCCCGGACTGGC235170.19366848899131714No Hit
TGAAAATCTTTGCTCTGAAGTGGGA232360.19135438237029576No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT216260.17809562201497744No Hit
GGATCCGGCCCGGACTGGC211450.17413446441814012No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC209900.17285799991188278No Hit
TGAAAATCTTTGCTCTGAAGTGGAG206760.17027212987985174No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC200770.165339212207283No Hit
GACCAAGATAGTCGAACTCGACCCTGTC198270.16328039848751308No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT195800.1612462905323804No Hit
CACCCCTCATCCGCTACTTTGACCTCATT193140.15905571273454522No Hit
TATTAATGCTGTAGGTGAAAGACC191170.1574333675233665No Hit
CTTTCCGAGGTCGCGGTC190000.15646984270251418No Hit
TATTAATGCTGTAGGTGAAAGACA189230.15583572807682505No Hit
TTCCGAGGTCGCGGTCGGACCGGGAAGG188910.1555721999206945No Hit
TGAAAATCTTTGCTCTGAGGTGGAA187520.1544274994925024No Hit
TTCCGAGGTCGCGGTCGGACCGGGA173000.1424699094080787No Hit
TTGGATCCGGCCCGGACTGGCC166630.13722405205010493No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAA165670.1364334675817133No Hit
CGGACTGGCCGTGGTCGAGGTGA165630.13640052656219698No Hit
CCGAGGTCGCGGTCGGACCGGGAAG164250.13526406138888397No Hit
TGAAAATCTTTGCTCTGGAGTGGAA162290.13364995143258435No Hit
TGGAAATCTTTGCTCTGAAGTGGAA160160.13189584214334038No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA152300.12542293180838374No Hit
CGGACTGGCCGTGGTCGAGGTGAGG149590.12319117773615312No Hit
CGAAAATCTTTGCTCTGAAGTGGAA147310.12131353962372296No Hit
GACCAAGATAGTCGAACTCGACCCTGTT146450.12060530770412212No Hit
TGAAAATCTTTGCCCTGAAGTGGAA145300.11965825339302794No Hit
TTCCGAGGTCGCGGTCGGACC141840.11680885520486638No Hit
TGAAGATCTTTGCTCTGAAGTGGAA141740.11672650265607558No Hit
GTGTGTGATGACTGCATAGTTCAACTGAACA136560.1124606406287123No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAAA135480.1115712331017717No Hit
TGAAAACCTTTGCTCTGAAGTGGAA133810.11019594553696539No Hit
TCGTTTATGCCGTGAGGATTGGAGGAAA132380.10901830408925699No Hit
TCCCTTTCCGAGGTCGCGGTC131090.10795595620985571No Hit
TGAAAATCTTTGCTCCGAAGTGGAA130040.10709125444755233No Hit
TATTAATGCTGTAGGTGAAAGACCA128390.10573243739250418No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAA127120.10468656002286106No Hit
TATAGACTTGGAAGCGGGAAAAAAAAAAAA126270.10398656335813929No Hit
GGTTGGATCCGGCCCGGACTGGC124690.1026853930872447No Hit
TGAAAATCTTTACTCTGAAGTGGAA122060.10051952105404674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)